Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1995-10-12
pubmed:abstractText
We have previously shown that efficient excision of the upstream large intron from P4-generated pre-mRNA of the autonomous parvovirus minute virus of mice depends upon at least the initial presence of sequences within the downstream small intron (Q. Zhao, R. V. Schoborg, and D. J. Pintel, J. Virol. 68:2849-2859, 1994). In this report, we show that the requirement of downstream small intron sequences is complex and that efficient excision of the upstream intron requires at least one small intron donor and the 3' splice site. In the absence of both small intron donors, a new spliced product is produced in which the intervening exon is skipped and the large intron donor at nucleotide 514 is joined to a small intron acceptor. Exon skipping caused by the loss of the two small intron donors can be overcome, and the excision of the large intron can be regained by mutations that improve the large intron polypyrimidine tract. These results are consistent with a model in which the binding of multiple splicing factors that assemble at both a downstream donor and acceptor facilitates the binding of splicing factors to the weak polypyrimidine tract of the upstream large intron, thereby defining the intervening exon and promoting excision of the upstream intron.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7666519-1280322, http://linkedlifedata.com/resource/pubmed/commentcorrection/7666519-1285125, http://linkedlifedata.com/resource/pubmed/commentcorrection/7666519-1316457, http://linkedlifedata.com/resource/pubmed/commentcorrection/7666519-1335742, http://linkedlifedata.com/resource/pubmed/commentcorrection/7666519-1592259, http://linkedlifedata.com/resource/pubmed/commentcorrection/7666519-1825251, http://linkedlifedata.com/resource/pubmed/commentcorrection/7666519-1825520, http://linkedlifedata.com/resource/pubmed/commentcorrection/7666519-2001841, http://linkedlifedata.com/resource/pubmed/commentcorrection/7666519-2063195, http://linkedlifedata.com/resource/pubmed/commentcorrection/7666519-2067018, http://linkedlifedata.com/resource/pubmed/commentcorrection/7666519-2136768, http://linkedlifedata.com/resource/pubmed/commentcorrection/7666519-2147041, http://linkedlifedata.com/resource/pubmed/commentcorrection/7666519-2164605, http://linkedlifedata.com/resource/pubmed/commentcorrection/7666519-2210374, http://linkedlifedata.com/resource/pubmed/commentcorrection/7666519-2398885, http://linkedlifedata.com/resource/pubmed/commentcorrection/7666519-2427200, http://linkedlifedata.com/resource/pubmed/commentcorrection/7666519-2694943, http://linkedlifedata.com/resource/pubmed/commentcorrection/7666519-2840206, http://linkedlifedata.com/resource/pubmed/commentcorrection/7666519-2939261, http://linkedlifedata.com/resource/pubmed/commentcorrection/7666519-2942705, http://linkedlifedata.com/resource/pubmed/commentcorrection/7666519-2943217, http://linkedlifedata.com/resource/pubmed/commentcorrection/7666519-3296697, http://linkedlifedata.com/resource/pubmed/commentcorrection/7666519-3346950, http://linkedlifedata.com/resource/pubmed/commentcorrection/7666519-3502703, http://linkedlifedata.com/resource/pubmed/commentcorrection/7666519-3658675, http://linkedlifedata.com/resource/pubmed/commentcorrection/7666519-3660591, http://linkedlifedata.com/resource/pubmed/commentcorrection/7666519-3783817, http://linkedlifedata.com/resource/pubmed/commentcorrection/7666519-4075405, http://linkedlifedata.com/resource/pubmed/commentcorrection/7666519-6828378, http://linkedlifedata.com/resource/pubmed/commentcorrection/7666519-7637034, http://linkedlifedata.com/resource/pubmed/commentcorrection/7666519-7852296, http://linkedlifedata.com/resource/pubmed/commentcorrection/7666519-7958840, http://linkedlifedata.com/resource/pubmed/commentcorrection/7666519-8151756, http://linkedlifedata.com/resource/pubmed/commentcorrection/7666519-8212598, http://linkedlifedata.com/resource/pubmed/commentcorrection/7666519-8340391, http://linkedlifedata.com/resource/pubmed/commentcorrection/7666519-8355683
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
69
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6170-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Efficient excision of the upstream large intron from P4-generated pre-mRNA of the parvovirus minute virus of mice requires at least one donor and the 3' splice site of the small downstream intron.
pubmed:affiliation
Department of Molecular Microbiology and Immunology, University of Missouri School of Medicine, Columbia 65212, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't