Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
37
|
| pubmed:dateCreated |
1995-10-12
|
| pubmed:abstractText |
Our previous studies have characterized mesenchyme-derived proteins to identify biologically active proteins and novel markers for stromal cell paracrine action relative to stromal-epithelial interactions. Previous reports have characterized properties of a growth inhibitory activity (to bladder and prostatic epithelial cells), secreted by U4F fetal rat urogenital sinus mesenchymal cells, not cross-reactive with antibodies to known cytokines, and provisionally termed UGIF. The present study reports the characterization, purification, and biological properties of a 20-21-kDa protein responsible for UGIF activity. The 20-21-kDa protein (termed ps20) was purified to near homogeneity, the amino-terminal sequence was determined, and biological properties were characterized in vitro. Amino-terminal sequence analysis indicated no direct matches or regions of homology with known proteins. Purified ps20 induced a linear and saturable inhibition of [3H]thymidine incorporation in PC-3 prostatic carcinoma cells (half-maximal activity at 2.6 nM), inhibited cell proliferation (increased population doubling time from 19.8 to 25.8 h), and induced a 210% stimulation in the synthesis of secreted proteins. These data suggest that ps20 may be a candidate paracrine effector protein and may play a role in stromal-epithelial cell interaction in the prostate gland.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
270
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
22058-65
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:7665628-Animals,
pubmed-meshheading:7665628-Cell Division,
pubmed-meshheading:7665628-Cell Line,
pubmed-meshheading:7665628-Chromatography, Gel,
pubmed-meshheading:7665628-Chromatography, High Pressure Liquid,
pubmed-meshheading:7665628-Chromatography, Ion Exchange,
pubmed-meshheading:7665628-Connective Tissue,
pubmed-meshheading:7665628-Connective Tissue Cells,
pubmed-meshheading:7665628-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:7665628-Epithelial Cells,
pubmed-meshheading:7665628-Fetus,
pubmed-meshheading:7665628-Growth Inhibitors,
pubmed-meshheading:7665628-Male,
pubmed-meshheading:7665628-Molecular Weight,
pubmed-meshheading:7665628-Prostate,
pubmed-meshheading:7665628-Protein Biosynthesis,
pubmed-meshheading:7665628-Proteins,
pubmed-meshheading:7665628-Rats,
pubmed-meshheading:7665628-Urinary Bladder,
pubmed-meshheading:7665628-Urogenital System
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Purification of a novel protein (ps20) from urogenital sinus mesenchymal cells with growth inhibitory properties in vitro.
|
| pubmed:affiliation |
Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|