Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1995-10-10
|
| pubmed:abstractText |
In this study we tested whether the pattern of cytokines expressed by human carcinomas could account for a different in vivo recruitment of leukocyte subpopulations as a part of the anti-tumor immune response. Two carcinoma cell lines, SK-OV-3 ovary carcinoma and CALU-3 lung carcinoma, were analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR), immunofluorescence and ELISA for the expression and in vitro production of cytokines with chemotactic, proinflammatory and growth-stimulating activity. Although both cell lines displayed a constitutive expression of granulocyte colony-stimulating factor (G-CSF), granulocyte macrophage-CSF (GM-CSF), M-CSF, interleukin (IL-) 1 alpha and IL-8, only CALU-3 cell line expressed IL-10, RANTES (Regulated upon Activation, Normal T Expressed and Secreted) and monocyte-activating protein (MCP)-1. MCP-1 and IL-8 were detected by immunohistochemistry on sections from tumors xenografted in nude mice. To analyze whether the tumor-released cytokines modulate leukocytes in tumor infiltration, we studied the distribution of human peripheral blood leukocytes injected in the proximity of SK-OV-3 and of CALU-3 tumor xenografts. While SK-OV-3 was unable to recruit human leukocytes and appeared to be barely infiltrated by murine CD45+ cells, CALU-3 appeared to be rapidly and heavily infiltrated by human leukocytes which induced tumor necrosis within 18-24 hr.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0020-7136
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
4
|
| pubmed:volume |
62
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
572-8
|
| pubmed:dateRevised |
2007-7-24
|
| pubmed:meshHeading |
pubmed-meshheading:7665228-Adenocarcinoma,
pubmed-meshheading:7665228-Animals,
pubmed-meshheading:7665228-Base Sequence,
pubmed-meshheading:7665228-Cytokines,
pubmed-meshheading:7665228-DNA Primers,
pubmed-meshheading:7665228-Female,
pubmed-meshheading:7665228-Humans,
pubmed-meshheading:7665228-Lung Neoplasms,
pubmed-meshheading:7665228-Lymphocytes, Tumor-Infiltrating,
pubmed-meshheading:7665228-Mice,
pubmed-meshheading:7665228-Mice, Nude,
pubmed-meshheading:7665228-Molecular Sequence Data,
pubmed-meshheading:7665228-Neoplasm Transplantation,
pubmed-meshheading:7665228-Ovarian Neoplasms,
pubmed-meshheading:7665228-RNA, Neoplasm,
pubmed-meshheading:7665228-Transplantation, Heterologous,
pubmed-meshheading:7665228-Tumor Cells, Cultured
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
An in vivo model to compare human leukocyte infiltration in carcinoma xenografts producing different chemokines.
|
| pubmed:affiliation |
Division of Experimental Oncology D, Istituto Nazionale Tumori, Milan, Italy.
|
| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
|