Linked Life Data

7665185

Source:http://linkedlifedata.com/resource/pubmed/id/7665185

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1995-10-12
pubmed:databankReference
pubmed:abstractText
The complete human BCR gene (152-141 nt) on chromosome 22 and greater than 80% of the human ABL gene (179-512 nt) on chromosome 9 have been sequenced from mapped cosmid and plasmid clones via a shotgun strategy. Because these two chromosomes are translocated with breakpoints within the BCR and ABL genes in Philadelphia chromosome-positive leukemias, knowledge of these sequences also might provide insight into the validity of various theories of chromosomal rearrangements. Comparison of these genes with their cDNA sequences reveal the positions of 23 BCR exons and putative alternative BCR first and second exons, as well as the common ABL exons 2-11, respectively. Additionally, these regions include the alternative ABL first exons 1b and 1a, a new gene 5' to the first ABL exon, and an open reading frame with homology to an EST within the BCR fourth intron. Further analysis reveals an Alu homology of 38.83 and 39.35% for the BCR and ABL genes, respectively, with other repeat elements present to a lesser extent. Four new Philadelphia chromosome translocation breakpoints from chronic myelogenous leukemia patients also were sequenced, and the positions of these and several other previously sequenced breakpoints now have been mapped precisely, although no consistent breakpoint features immediately were apparent. Comparative analysis of genomic sequences encompassing the murine homologues to the human ABL exons 1b and 1a, as well as regions encompassing the ABL exons 2 and 3, reveals that although there is a high degree of homology in their corresponding exons and promoter regions, these two vertebrate species show a striking lack of homology outside these regions.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0888-7543
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
27
pubmed:geneSymbol
ABL, BCR
pubmed:owner
NLM
pubmed:authorsComplete
N
pubmed:pagination
67-82
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:7665185-Animals, pubmed-meshheading:7665185-Base Sequence, pubmed-meshheading:7665185-Chromosomes, Human, Pair 22, pubmed-meshheading:7665185-Chromosomes, Human, Pair 9, pubmed-meshheading:7665185-DNA, Complementary, pubmed-meshheading:7665185-Genes, pubmed-meshheading:7665185-Genes, abl, pubmed-meshheading:7665185-Humans, pubmed-meshheading:7665185-Leukemia, Myelogenous, Chronic, BCR-ABL Positive, pubmed-meshheading:7665185-Mice, pubmed-meshheading:7665185-Minisatellite Repeats, pubmed-meshheading:7665185-Molecular Sequence Data, pubmed-meshheading:7665185-Oncogene Proteins, pubmed-meshheading:7665185-Philadelphia Chromosome, pubmed-meshheading:7665185-Protein-Tyrosine Kinases, pubmed-meshheading:7665185-Proto-Oncogene Proteins, pubmed-meshheading:7665185-Proto-Oncogene Proteins c-bcr, pubmed-meshheading:7665185-Sequence Alignment, pubmed-meshheading:7665185-Sequence Homology, Nucleic Acid, pubmed-meshheading:7665185-Species Specificity
pubmed:year
1995
pubmed:articleTitle
Sequence and analysis of the human ABL gene, the BCR gene, and regions involved in the Philadelphia chromosomal translocation.
pubmed:affiliation
University of Oklahoma, Department of Chemistry and Biochemistry, Norman 73019, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't