Linked Life Data

7664984

Source:http://linkedlifedata.com/resource/pubmed/id/7664984

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-10-10
pubmed:abstractText
Complex between urokinase and its type-1 inhibitor (uPA-PAI-1) may, when bound to the urokinase receptor (uPAR), be endocytosed by an ensuing binding of the complex to the multiligand receptors alpha (2)-macroglobulin receptor/low density lipoprotein receptor-related protein (alpha 2MR/LRP) and glycoprotein 330 (gp330). We have found that phorbol esters regulate endocytosis of uPA-PAI-1 differently in different cell lines. In COS-1 cells, expressing uPAR and high levels of alpha 2MR/LRP under basal conditions, phorbol esters cause a time-dependent decrease in endocytosis concomitantly with a parallel down-regulation of alpha 2MR/LRP expression. An up-regulation of uPAR expression was also observed. General endocytosis via the clathrin-coated pit pathway was not affected by PMA treatment, as judged from measurements of transferrin endocytosis. In LLC-PK1 cells, expressing alpha 2MR/LRP but not uPAR under basal conditions, phorbol esters transiently increase endocytosis in parallel with a transient induction of uPAR expression, while there was virtually no change in alpha 2MR/LRP expression. Differential regulation of endocytosis therefore seems to be caused by differential regulation of the receptors, with either the alpha 2MR/LRP-level (in COS)-1 cells) or the uPAR-level (in LLC-PK1 cells) being rate-limiting.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0303-7207
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
109
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
209-17
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:7664984-Animals, pubmed-meshheading:7664984-Cell Line, pubmed-meshheading:7664984-Cell Line, Transformed, pubmed-meshheading:7664984-Cercopithecus aethiops, pubmed-meshheading:7664984-Coated Pits, Cell-Membrane, pubmed-meshheading:7664984-Endocytosis, pubmed-meshheading:7664984-Gene Expression Regulation, pubmed-meshheading:7664984-Humans, pubmed-meshheading:7664984-Kidney, pubmed-meshheading:7664984-Low Density Lipoprotein Receptor-Related Protein-1, pubmed-meshheading:7664984-Plasminogen Activator Inhibitor 1, pubmed-meshheading:7664984-Receptors, Cell Surface, pubmed-meshheading:7664984-Receptors, Immunologic, pubmed-meshheading:7664984-Receptors, Urokinase Plasminogen Activator, pubmed-meshheading:7664984-Swine, pubmed-meshheading:7664984-Tetradecanoylphorbol Acetate, pubmed-meshheading:7664984-Urokinase-Type Plasminogen Activator
pubmed:year
1995
pubmed:articleTitle
Differential regulation of urokinase-type-1 inhibitor complex endocytosis by phorbol esters in different cell lines is associated with differential regulation of alpha 2-macroglobulin receptor and urokinase receptor expression.
pubmed:affiliation
Department of Molecular Biology, University of Aarhus, Denmark.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't