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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1995-10-6
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pubmed:abstractText |
Modified vaccinia virus Ankara (MVA), a host range restricted and highly attenuated vaccinia virus strain, is unable to multiply in human and most other mammalian cell lines. Since viral gene expression is unimpaired in non-permissive cells recombinant MVA viruses are efficient as well as exceptionally safe expression vectors. We constructed a recombinant MVA that expresses the bacteriophage T7 RNA polymerase and tested its usefulness for transient expression of recombinant genes under the control of a T7 promoter. Using the chloramphenicol acetyltransferase (CAT) gene as a reporter gene, infection with MVA-T7pol allowed efficient synthesis of recombinant enzyme in mammalian cells. Despite the severe host restriction of MVA, enzyme activities induced by infection with MVA-T7pol were similar to those determined after infection with a replication-competent vaccinia-T7pol recombinant virus. Thus, MVA-T7pol may be used as a novel vaccinia vector to achieve T7 RNA polymerase-specific recombinant gene expression in the absence of productive vaccinia virus replication.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
28
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pubmed:volume |
371
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9-12
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:7664891-Animals,
pubmed-meshheading:7664891-Base Sequence,
pubmed-meshheading:7664891-Cells, Cultured,
pubmed-meshheading:7664891-Chick Embryo,
pubmed-meshheading:7664891-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:7664891-DNA-Directed RNA Polymerases,
pubmed-meshheading:7664891-Fibroblasts,
pubmed-meshheading:7664891-Gene Expression Regulation, Viral,
pubmed-meshheading:7664891-Genes, Reporter,
pubmed-meshheading:7664891-Genetic Vectors,
pubmed-meshheading:7664891-Haplorhini,
pubmed-meshheading:7664891-HeLa Cells,
pubmed-meshheading:7664891-Humans,
pubmed-meshheading:7664891-Molecular Sequence Data,
pubmed-meshheading:7664891-Promoter Regions, Genetic,
pubmed-meshheading:7664891-Recombinant Fusion Proteins,
pubmed-meshheading:7664891-Vaccinia virus,
pubmed-meshheading:7664891-Viral Proteins,
pubmed-meshheading:7664891-Virus Replication,
pubmed-meshheading:7664891-beta-Galactosidase
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pubmed:year |
1995
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pubmed:articleTitle |
Non-replicating vaccinia vector efficiently expresses bacteriophage T7 RNA polymerase.
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pubmed:affiliation |
Institut für Molekulare Virologie, GSF-Forschungszentrum für Unwelt und Gesundheit GmbH, Oberschleissheim, FRG.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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