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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
1995-10-10
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pubmed:abstractText |
Cell cycle-dependence of parathyroid hormone (PTH)-induced signal transduction was studied at the single cell level in fetal rat osteoblast-like cells (ROB) in primary culture. Responsiveness to 10(-7) M rPTH1-34 was measured as changes in the intracellular calcium concentration ([Ca2+]i) of cells loaded with Fura-2 using a video imaging setup. Cells in S-phase were identified by staining for incorporated 5-bromo-2'-deoxyuridine (BrdU). ROB were cultured on glass coverslips which had alphanumerically marked gratings so that cells could be located. Directly after measurement of the PTH-induced [Ca2+]i response of the individual cells, cells were fixed and stained for incorporated BrdU. Phase-contrast images taken with the video imaging setup were compared with phase-contrast micrographs and fluoro-micrographs taken after staining for BrdU incorporation. We found that 43% of the cells responding to PTH with a rise in [Ca2+]i had also incorporated BrdU. This percentage was not different from the percentage BrdU positive cells in the whole culture, showing that calcium responsiveness was randomly distributed between ROB in S-phase and those not in S-phase. We therefore conclude that PTH does not induce calcium responses preferentially during the S-phase of the cell cycle in ROB in primary culture.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0143-4160
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
270-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7664314-Animals,
pubmed-meshheading:7664314-Calcium,
pubmed-meshheading:7664314-Cells, Cultured,
pubmed-meshheading:7664314-DNA Replication,
pubmed-meshheading:7664314-Osteoblasts,
pubmed-meshheading:7664314-Parathyroid Hormone,
pubmed-meshheading:7664314-Peptide Fragments,
pubmed-meshheading:7664314-Rats,
pubmed-meshheading:7664314-S Phase,
pubmed-meshheading:7664314-Signal Transduction,
pubmed-meshheading:7664314-Skull,
pubmed-meshheading:7664314-Teriparatide
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pubmed:year |
1995
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pubmed:articleTitle |
S-phase independence of parathyroid hormone-induced calcium signalling in primary osteoblast-like cells.
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pubmed:affiliation |
Department of Physiology and Physiological Physics, Leiden University, The Netherlands.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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