Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1995-10-12
pubmed:abstractText
Here we describe a system that enables short peptides to bind DNA sequence-specifically. Linking the peptide covalently to DNA through a disulphide bond eliminates the unfavourable energetic cost of diffusion and thus potentiates the peptide-DNA interaction. By this approach we have deconstructed the GCN4/DNA complex into its elemental DNA recognition units. We find that the GCN4 basic region contacts the two half-sites with very different affinities and propose that this thermodynamic asymmetry plays a role in differential regulation of gene expression. Specific binding of the peptide to DNA stabilizes the disulphide bond toward reduction suggesting a novel approach to the discovery of new DNA-binding specificities.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1072-8368
pubmed:author
pubmed:issnType
Print
pubmed:volume
2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
450-7
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:7664107-Amino Acid Sequence, pubmed-meshheading:7664107-Base Sequence, pubmed-meshheading:7664107-Circular Dichroism, pubmed-meshheading:7664107-Consensus Sequence, pubmed-meshheading:7664107-DNA, pubmed-meshheading:7664107-DNA-Binding Proteins, pubmed-meshheading:7664107-Disulfides, pubmed-meshheading:7664107-Fungal Proteins, pubmed-meshheading:7664107-Leucine Zippers, pubmed-meshheading:7664107-Models, Chemical, pubmed-meshheading:7664107-Models, Molecular, pubmed-meshheading:7664107-Molecular Sequence Data, pubmed-meshheading:7664107-Molecular Structure, pubmed-meshheading:7664107-Peptides, pubmed-meshheading:7664107-Protein Binding, pubmed-meshheading:7664107-Protein Kinases, pubmed-meshheading:7664107-Saccharomyces cerevisiae Proteins, pubmed-meshheading:7664107-Structure-Activity Relationship, pubmed-meshheading:7664107-Sulfhydryl Reagents, pubmed-meshheading:7664107-Temperature
pubmed:year
1995
pubmed:articleTitle
Deconstruction of GCN4/GCRE into a monomeric peptide-DNA complex.
pubmed:affiliation
Department of Chemistry, Harvard University, Cambridge, Massachusetts 02138, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't