rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
6
|
pubmed:dateCreated |
1995-10-12
|
pubmed:abstractText |
Here we describe a system that enables short peptides to bind DNA sequence-specifically. Linking the peptide covalently to DNA through a disulphide bond eliminates the unfavourable energetic cost of diffusion and thus potentiates the peptide-DNA interaction. By this approach we have deconstructed the GCN4/DNA complex into its elemental DNA recognition units. We find that the GCN4 basic region contacts the two half-sites with very different affinities and propose that this thermodynamic asymmetry plays a role in differential regulation of gene expression. Specific binding of the peptide to DNA stabilizes the disulphide bond toward reduction suggesting a novel approach to the discovery of new DNA-binding specificities.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Jun
|
pubmed:issn |
1072-8368
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
2
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
450-7
|
pubmed:dateRevised |
2009-11-19
|
pubmed:meshHeading |
pubmed-meshheading:7664107-Amino Acid Sequence,
pubmed-meshheading:7664107-Base Sequence,
pubmed-meshheading:7664107-Circular Dichroism,
pubmed-meshheading:7664107-Consensus Sequence,
pubmed-meshheading:7664107-DNA,
pubmed-meshheading:7664107-DNA-Binding Proteins,
pubmed-meshheading:7664107-Disulfides,
pubmed-meshheading:7664107-Fungal Proteins,
pubmed-meshheading:7664107-Leucine Zippers,
pubmed-meshheading:7664107-Models, Chemical,
pubmed-meshheading:7664107-Models, Molecular,
pubmed-meshheading:7664107-Molecular Sequence Data,
pubmed-meshheading:7664107-Molecular Structure,
pubmed-meshheading:7664107-Peptides,
pubmed-meshheading:7664107-Protein Binding,
pubmed-meshheading:7664107-Protein Kinases,
pubmed-meshheading:7664107-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:7664107-Structure-Activity Relationship,
pubmed-meshheading:7664107-Sulfhydryl Reagents,
pubmed-meshheading:7664107-Temperature
|
pubmed:year |
1995
|
pubmed:articleTitle |
Deconstruction of GCN4/GCRE into a monomeric peptide-DNA complex.
|
pubmed:affiliation |
Department of Chemistry, Harvard University, Cambridge, Massachusetts 02138, USA.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|