Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1995-10-12
|
| pubmed:abstractText |
The occurrence of a large-cell lymphoma (LCL) concurrent with or subsequent to lymphocytic predominance Hodgkin's disease (LPHD) is well documented. Given the well-characterized B-cell nature of the Reed-Sternberg cell variants in LPHD, there may be a clonal relationship between the LPHD and the associated B-cell LCL. In this study, we adapted a highly sensitive, clonospecific assay to test whether the clone comprising the LCL exists in the corresponding LPHD tumor. Nine cases meeting the histologic criteria of nodular LPHD and B-cell LCL were identified, reviewed, and studied. Initially, clonality of both lesions was assessed using consensus primers to conserved regions in the IgH variable (frame-work III) and joining region genes in a polymerase chain reaction (PCR) assay. The PCR assay detected a clonal B-cell population in five of the LCLs, whereas analysis of eight cases of LPHD did not detect a dominant clone. Clonal products from the LCL were then sequenced, and clonospecific oligonucleotides were designed from the unique nucleotide sequence encoding the complementarity-determining region-III. These were then used as primers and/or probes in sensitive PCR-based assays on the corresponding LPHD tumors. In two cases, the clonospecific assay showed that the LPHD and LCL shared a common clone that was further confirmed by sequence analysis. This finding provides genotypic evidence that, at least in some cases, the LCL represents a clonal progression of LPHD.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0006-4971
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
86
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2312-20
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7662978-Adult,
pubmed-meshheading:7662978-Aged,
pubmed-meshheading:7662978-B-Lymphocytes,
pubmed-meshheading:7662978-Base Sequence,
pubmed-meshheading:7662978-Clone Cells,
pubmed-meshheading:7662978-DNA, Neoplasm,
pubmed-meshheading:7662978-Disease Progression,
pubmed-meshheading:7662978-Female,
pubmed-meshheading:7662978-Hodgkin Disease,
pubmed-meshheading:7662978-Humans,
pubmed-meshheading:7662978-Lymphoma, B-Cell,
pubmed-meshheading:7662978-Lymphoma, Large B-Cell, Diffuse,
pubmed-meshheading:7662978-Male,
pubmed-meshheading:7662978-Middle Aged,
pubmed-meshheading:7662978-Molecular Sequence Data,
pubmed-meshheading:7662978-Neoplasms, Multiple Primary,
pubmed-meshheading:7662978-Polymerase Chain Reaction,
pubmed-meshheading:7662978-Reed-Sternberg Cells,
pubmed-meshheading:7662978-Sequence Alignment,
pubmed-meshheading:7662978-Sequence Homology, Nucleic Acid
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Clonal relationship between lymphocytic predominance Hodgkin's disease and concurrent or subsequent large-cell lymphoma of B lineage.
|
| pubmed:affiliation |
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha 68198-3135, USA.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
|