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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1995-10-3
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pubmed:abstractText |
1. Whole-cell and intracellular recordings were made from neurons in slices of guinea-pig spinal trigeminal nucleus pars caudalis. 2. 5-Hydroxytryptamine (5-HT) hyperpolarized 70% of neurons by activating 5-HT1A receptors. The effect was mimicked by 5-carboxamidotryptamine (5-CT) and (+/-)-2-dipropylamino-8-hydroxy-1,2,3,4-tetrahydronapthalene hydrobromide (8-OH-DPAT) and antagonized by 1-(2-methoxyphenyl)-4-[4-(2-phthalimido)-butyl]-piperazine hydrobromide (NAN 190) and pindobind-5-HT1A. Nine per cent of the neurons were depolarized by 5-HT. 3. In about 20% of recordings, 5-HT also evoked repetitive inhibitory postsynaptic potentials that were mediated by glycine. 4. Noradrenaline (NA) hyperpolarized 71% of neurons. This effect was mediated by activation of alpha 2-adrenoceptors, since 5-bromo-N-(4,5-dihydro-1H-imidazol-2-yl)-6-quinoxalinamine (UK14304) also caused a hyperpolarization and idazoxan (1 microM) blocked the hyperpolarization to both NA and UK14304. Phenylephrine depolarized a subset of neurons and this depolarization was blocked by prazosin, suggesting an action mediated by activation of alpha 1-adrenoceptors. 5. NA also evoked repetitive GABAA-mediated inhibitory postsynaptic potentials in about 20% of recordings. The increase in synaptic activity was mimicked by phenylephrine and blocked by prazosin. 6. These results indicate that there are at least two mechanisms through which 5-HT and NA inhibit neurons: (i) in many cells both 5-HT and NA mediate a hyperpolarization through an increase of a potassium conductance; (ii) 5-HT and NA also activated GABA- and glycine-containing interneurons to cause IPSPs in separate groups of cells.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7658366-1000295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7658366-1354394,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7658366-14067768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7658366-1578363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7658366-1832328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7658366-1884202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7658366-2087262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7658366-2141079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7658366-2175924,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7658366-2337803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7658366-2358549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7658366-2370323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7658366-2566681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7658366-2861606,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7658366-2868781,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7658366-2872283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7658366-2901110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7658366-2996695,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7658366-3025380,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/7658366-3427477,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/7658366-8381444
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-3751
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
485 ( Pt 1)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
113-20
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:7658366-Adrenergic alpha-1 Receptor Agonists,
pubmed-meshheading:7658366-Adrenergic alpha-2 Receptor Agonists,
pubmed-meshheading:7658366-Animals,
pubmed-meshheading:7658366-Guinea Pigs,
pubmed-meshheading:7658366-Male,
pubmed-meshheading:7658366-Membrane Potentials,
pubmed-meshheading:7658366-Neurons,
pubmed-meshheading:7658366-Nociceptors,
pubmed-meshheading:7658366-Norepinephrine,
pubmed-meshheading:7658366-Potassium Channels,
pubmed-meshheading:7658366-Quinoxalines,
pubmed-meshheading:7658366-Receptors, GABA-A,
pubmed-meshheading:7658366-Serotonin,
pubmed-meshheading:7658366-Serotonin Receptor Agonists,
pubmed-meshheading:7658366-Substantia Gelatinosa,
pubmed-meshheading:7658366-Synapses,
pubmed-meshheading:7658366-Trigeminal Nucleus, Spinal
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pubmed:year |
1995
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pubmed:articleTitle |
Inhibition by 5-hydroxytryptamine and noradrenaline in substantia gelatinosa of guinea-pig spinal trigeminal nucleus.
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pubmed:affiliation |
Vollum Institute, Oregon Health Sciences University, Portland 97201, USA.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.
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