Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
35
|
| pubmed:dateCreated |
1995-10-4
|
| pubmed:abstractText |
Tumors obtained from v-Ha-ras-transformed PB-3c cells are characterized by autocrine interleukin-3 (IL3) expression, which occurs either without (class I tumors) or with enhanced transcription (class II tumors). To address possible post-transcriptional mechanisms of IL3 expression, IL3 mRNA stability was examined in both tumor classes. Increased stability of IL3 mRNA was detected in class I tumor lines (t1/2 > 3 h), whereas rapid decay of IL3 transcripts (t1/2 < 0.5 h) was found in class II tumor lines. In both tumor classes, the c-myc and interleukin-6 transcripts were short-lived. Transcripts of a constitutively expressed IL3 reporter gene were stable in class I tumor cells but unstable in class II tumor cells, suggesting that IL3 mRNA stabilization involved a trans-acting mechanism. Rapid decay of IL3 reporter transcripts was observed in untransformed PB-3c as well as in v-Ha-ras expressing precursor cells linking transcript stabilization to the tumor stage. Reporter transcript stabilization in class I tumor cells correlated with increased IL3 production. Deletion of the AU-rich element from the IL3 reporter gene further augmented IL3 mRNA levels as well as IL3 production, suggesting that the stabilizing mechanism in class I tumor cells is not equivalent to AU-rich element deletion.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Dactinomycin,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-3,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Thymidine
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
270
|
| pubmed:geneSymbol |
v-Ha-ras
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
20629-35
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:7657642-Animals,
pubmed-meshheading:7657642-Base Sequence,
pubmed-meshheading:7657642-Cell Line, Transformed,
pubmed-meshheading:7657642-Cell Transformation, Neoplastic,
pubmed-meshheading:7657642-DNA Primers,
pubmed-meshheading:7657642-DNA Replication,
pubmed-meshheading:7657642-Dactinomycin,
pubmed-meshheading:7657642-Gene Rearrangement,
pubmed-meshheading:7657642-Genes, ras,
pubmed-meshheading:7657642-Interleukin-3,
pubmed-meshheading:7657642-Kinetics,
pubmed-meshheading:7657642-Mast Cells,
pubmed-meshheading:7657642-Mice,
pubmed-meshheading:7657642-Mice, Inbred DBA,
pubmed-meshheading:7657642-Molecular Sequence Data,
pubmed-meshheading:7657642-Neoplasms, Experimental,
pubmed-meshheading:7657642-Polymerase Chain Reaction,
pubmed-meshheading:7657642-RNA, Messenger,
pubmed-meshheading:7657642-RNA, Neoplasm,
pubmed-meshheading:7657642-Thymidine,
pubmed-meshheading:7657642-Transcription, Genetic,
pubmed-meshheading:7657642-Transcriptional Activation,
pubmed-meshheading:7657642-Transfection
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Interleukin-3 mRNA stabilization by a trans-acting mechanism in autocrine tumors lacking interleukin-3 gene rearrangements.
|
| pubmed:affiliation |
Institute for Medical Microbiology, University of Basel, Switzerland.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|