Linked Life Data

7654240

Source:http://linkedlifedata.com/resource/pubmed/id/7654240

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1995-9-25
pubmed:databankReference
pubmed:abstractText
We have screened a lambda gt11 cDNA library from chicken gizzard muscle with polyclonal antiserum to avian synemin. Immunopositive clones were characterized and sequenced. Computer searches identified primarily intermediate filament (IF) proteins as being homologous to the synemin clones. Synemin's sequence contained all structural features characteristic of the rod domain of IF proteins: (a) its length (approximately 310 amino acids), (b) the heptad repeat pattern of hydrophobic residues of coiled-coil proteins, (c) subdomain structure of the IF rod, by which helical subdomains (1A, 1B, 2A, and 2B) are separated by three short non-helical linker regions, and (d) several potential intrahelical ion pairs along the sequence. We also confirmed the presence of the IF rod domain at the protein level by immunoblotting a proteolytic digest of synemin by using a monoclonal antibody specific to the rod domain of IFs.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
24
pubmed:volume
213
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
796-802
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Synemin contains the rod domain of intermediate filaments.
pubmed:affiliation
Department of Biochemistry, Iowa State University, Ames 50011, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't