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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
|
| pubmed:dateCreated |
1995-9-26
|
| pubmed:abstractText |
This study evaluated the effect of varying the synthesis of nitric oxide with sodium nitroprusside or N-nitro-L-arginine methyl ester (L-NAME) in a pancreatitis-lung injury model. Rats (n = 45) were randomized to control or caerulein-induced pancreatitis groups, treated with saline, sodium nitroprusside (0.4 micrograms/kg) or L-NAME (10 mg/kg). Myeloperoxidase activity was used as a measure of neutrophil infiltration. Wet to dry (W:D) lung weight and bronchoalveolar lavage (BAL) protein concentrations were used to assess vascular leakage. Pancreatitis was shown to induce pulmonary neutrophil influx: mean(s.e.m.) myeloperoxidase activity 6.79(0.5) units/g in caerulein-treated animals versus 2.08(0.5) units/g in controls (P < 0.001). Animals with pancreatitis showed increased microvascular leakage compared with controls (mean(s.e.m.) W:D lung weight 7.01(0.5) versus 2.85(0.2), P < 0.001; BAL protein concentration 2539(222) versus 347(32) micrograms/ml, P < 0.001). Compared with the saline-treated pancreatitis group, these changes were reduced by sodium nitroprusside (mean(s.e.m.) myeloperoxidase activity to 2.5(0.4) units/g, P < 0.001; W:D lung weight to 3.8(0.37), P < 0.001; BAL protein concentration 1389(182) micrograms/ml, P < 0.05). L-NAME exacerbated the pancreatitis-induced pulmonary oedema (W:D lung weight increased to 11.96(0.6), P < 0.001), protein leakage (BAL protein concentration rose to 3707(309) micrograms/ml, P < 0.05) and neutrophil infiltration (myeloperoxidase activity increased to 9.01(0.3) units/g, P < 0.05). These data suggest that, in vivo, nitric oxide inhibits pancreatitis-induced lung injury, possibly in part by inhibiting pulmonary neutrophil influx.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0007-1323
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
82
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1122-6
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:7648171-Acute Disease,
pubmed-meshheading:7648171-Animals,
pubmed-meshheading:7648171-Bronchoalveolar Lavage Fluid,
pubmed-meshheading:7648171-Disease Models, Animal,
pubmed-meshheading:7648171-Lung,
pubmed-meshheading:7648171-Lung Diseases,
pubmed-meshheading:7648171-Male,
pubmed-meshheading:7648171-Nitric Oxide,
pubmed-meshheading:7648171-Organ Size,
pubmed-meshheading:7648171-Pancreatitis,
pubmed-meshheading:7648171-Peroxidase,
pubmed-meshheading:7648171-Proteins,
pubmed-meshheading:7648171-Rats,
pubmed-meshheading:7648171-Rats, Sprague-Dawley
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Role of nitric oxide in lung injury associated with experimental acute pancreatitis.
|
| pubmed:affiliation |
Department of Surgery, Royal College of Surgeons in Ireland, Beaumont Hospital, Dublin.
|
| pubmed:publicationType |
Journal Article
|