Linked Life Data

7645031

Source:http://linkedlifedata.com/resource/pubmed/id/7645031

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-9-21
pubmed:abstractText
Fumonisins are responsible for at least two diseases of veterinary importance (equine leukoencephalomalacia and porcine pulmonary edema) and are carcinogenic for experimental animals and, perhaps, humans. They have been found to disrupt sphingolipid metabolism in many types of cells, including hepatocytes, neurons, and renal cells. In this study, endothelial cells form porcine pulmonary arteries were cultured on micropore filters as a model for the endothelial barrier, and barrier function was quantitated as the movement of albumin across the endothelial monolayers. Fumonisin B1 increased the amount of free sphinganine by 20- to 30-fold within 3 hr, as expected for inhibition of sphinganine (sphingosine) N-acyltransferase by this mycotoxin. At 30 to 50 microM, fumonisin B1 doubled the rate of albumin transfer across endothelial monolayers; however, there was no loss of cell viability based on morphology or trypan blue exclusion. When 15 microM D-erythro-sphinganine was added to the cells, the rate of albumin transfer also doubled (after 24 hr incubation) without a loss of viability; however, this treatment increased the cellular level of sphinganine by > 100-fold. Addition of 25 microM sphinganine caused even greater albumin transfer, but also resulted in significant cell death. These results establish that fumonisin B1 and D-erythro-sphinganine allow accelerated passage of macromolecules across the endothelium. Fumonisin B1 alters sphingolipid biosynthesis with an elevation of sphinganine in the cells which may, at least in part, explain the observed disruption of endothelial barrier function.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0041-008X
pubmed:author
pubmed:issnType
Print
pubmed:volume
133
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
343-8
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Fumonisin B1 alters sphingolipid metabolism and disrupts the barrier function of endothelial cells in culture.
pubmed:affiliation
Department of Nutrition and Food Science, University of Kentucky, Lexington, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.