Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1995-9-21
pubmed:abstractText
Enteroaggregative Escherichia coli (EAggEc) strains are associated with persistent diarrhea in children in the developing world and exhibit a classic aggregative phenotype. We have demonstrated that EAggEc strains isolated from children with persistent diarrhea in Brazil, Bangladesh, and Pakistan also have the potential to be internalized by HeLa cells in the gentamicin protection assay. We have confirmed this phenomenon with transmission electron micrographs of bacteria engulfed by HeLa cells. We examined the mechanisms by which this process occurs. Staurosporine inhibited internalization of EAggEc strain 162 by 50% at a concentration of 0.1 microM. Genistein inhibited internalization of this same organism by 50% at a concentration of 50 microM. Cytochalasin D inhibited internalization by 50% at a concentration of 1 microgram/ml. Staurosporine, genistein, and cytochalasin D inhibited the internalization of EAggEc strain 162 by HeLa cells in a dose-dependent manner. These data suggest that active cell processes such as signal transduction by protein kinase and/or tyrosine kinase may be involved in the internalization of EAggEc strain 162 by HeLa cells and that actin filaments and cytoskeletal structure may be important for this process.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7642271-1347759, http://linkedlifedata.com/resource/pubmed/commentcorrection/7642271-1350273, http://linkedlifedata.com/resource/pubmed/commentcorrection/7642271-1370017, http://linkedlifedata.com/resource/pubmed/commentcorrection/7642271-1396556, http://linkedlifedata.com/resource/pubmed/commentcorrection/7642271-1398907, http://linkedlifedata.com/resource/pubmed/commentcorrection/7642271-1452364, http://linkedlifedata.com/resource/pubmed/commentcorrection/7642271-1587588, http://linkedlifedata.com/resource/pubmed/commentcorrection/7642271-1653253, http://linkedlifedata.com/resource/pubmed/commentcorrection/7642271-1658721, http://linkedlifedata.com/resource/pubmed/commentcorrection/7642271-1671111, http://linkedlifedata.com/resource/pubmed/commentcorrection/7642271-1909337, http://linkedlifedata.com/resource/pubmed/commentcorrection/7642271-2010554, http://linkedlifedata.com/resource/pubmed/commentcorrection/7642271-2189007, http://linkedlifedata.com/resource/pubmed/commentcorrection/7642271-2569162, http://linkedlifedata.com/resource/pubmed/commentcorrection/7642271-2656875, http://linkedlifedata.com/resource/pubmed/commentcorrection/7642271-2668303, http://linkedlifedata.com/resource/pubmed/commentcorrection/7642271-2899125, http://linkedlifedata.com/resource/pubmed/commentcorrection/7642271-3106339, http://linkedlifedata.com/resource/pubmed/commentcorrection/7642271-3313248, http://linkedlifedata.com/resource/pubmed/commentcorrection/7642271-381204, http://linkedlifedata.com/resource/pubmed/commentcorrection/7642271-3900672, http://linkedlifedata.com/resource/pubmed/commentcorrection/7642271-7844392, http://linkedlifedata.com/resource/pubmed/commentcorrection/7642271-8094379, http://linkedlifedata.com/resource/pubmed/commentcorrection/7642271-8108852
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
63
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3417-21
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Characterization of an invasive phenotype associated with enteroaggregative Escherichia coli.
pubmed:affiliation
Department of Medicine, New England Deaconess Hospital, Boston, Massachusetts 02215, USA.
pubmed:publicationType
Journal Article