Linked Life Data

7642162

Source:http://linkedlifedata.com/resource/pubmed/id/7642162

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-9-21
pubmed:abstractText
Rat duodenum was examined for light and electron microscopical localization of carbonic anhydrase (CA) activity by the histochemical Hansson's method. The absorptive epithelial cells and some goblet cells showed a distinct reaction for CA activity in the cytoplasm, and on the striated borders and lateral cell borders. Electron microscopy confirmed that the reaction precipitates for CA activity are intimately associated with the cytoplasmic side of the cell membranes on both the lateral cell infoldings and the microvilli composing the brush borders. But the epithelial cells on the crypts were not positive except for some unidentified cells which show the distinct reaction. The duodenum was divided into four segments from the proximal to the distal part, and the homogenized mucosa scraped from each segment were examined for CA activity by Maren's physiological method. It was found that the activity decreased from the proximal to the distal segments. The most proximal segment showed the highest activity, 10.4 +/- 2.1 UA/mg of protein and 1468.7 +/- 324.8 UA/g of wet weight, and the most distal segment of duodenum showed the lowest activity, 2.4 +/- 0.7 UA/mg of protein and 291.7 +/- 85.8 UA/g of wet weight. The significance of the localization of CA activity in the villous epithelium of rat duodenum was discussed in relation to the bicarbonate section.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0016-2590
pubmed:author
pubmed:issnType
Print
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
103-17
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Histochemical localization of carbonic anhydrase in the rat duodenal epithelium.
pubmed:affiliation
Department of Anatomy II, Fukushima Medical College, Japan.
pubmed:publicationType
Journal Article