Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-9-21
pubmed:databankReference
pubmed:abstractText
The RAD51 gene is a eukaryotic counterpart of the Escherichia coli recA gene which is involved in genetic recombination. Two distinct Xenopus laevis RAD51 cDNA clones (XRAD51.1 and XRAD51.2) were isolated from an oocyte cDNA library using the human RAD51 cDNA (HsRAD51) as a probe. Sequence analysis revealed that 98.2% of the amino-acid residues were identical between XRAD51.1 and XRAD51.2, and that both were 95% identical to HsRAD51. Both of the XRAD51 genes were expressed at a higher level in ovary and testis than in other somatic tissues, suggesting their involvement in meiotic recombination. The expression of XRAD51.1 was about eightfold in excess of that of XRAD51.2 in all of the tissues examined. Analysis of the rates of synonymous substitution in the coding sequences of the two XRAD51 suggests that these two genes diverged about 50 million years ago. The structural similarities of the XRAD51 proteins to RecA in E. coli and Rad51 in yeasts or vertebrates are discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
160
pubmed:geneSymbol
HsRAD51, RAD51, XRAD51.1, XRAD51.2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
195-200
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:7642095-Amino Acid Sequence, pubmed-meshheading:7642095-Animals, pubmed-meshheading:7642095-Base Sequence, pubmed-meshheading:7642095-DNA, Complementary, pubmed-meshheading:7642095-DNA-Binding Proteins, pubmed-meshheading:7642095-Female, pubmed-meshheading:7642095-Genes, pubmed-meshheading:7642095-Male, pubmed-meshheading:7642095-Mammals, pubmed-meshheading:7642095-Meiosis, pubmed-meshheading:7642095-Molecular Sequence Data, pubmed-meshheading:7642095-Multigene Family, pubmed-meshheading:7642095-Organ Specificity, pubmed-meshheading:7642095-Ovary, pubmed-meshheading:7642095-Phylogeny, pubmed-meshheading:7642095-Rad51 Recombinase, pubmed-meshheading:7642095-Recombination, Genetic, pubmed-meshheading:7642095-Sequence Alignment, pubmed-meshheading:7642095-Sequence Homology, pubmed-meshheading:7642095-Species Specificity, pubmed-meshheading:7642095-Testis, pubmed-meshheading:7642095-Xenopus Proteins, pubmed-meshheading:7642095-Xenopus laevis
pubmed:year
1995
pubmed:articleTitle
RAD51 homologues in Xenopus laevis: two distinct genes are highly expressed in ovary and testis.
pubmed:affiliation
Department of Molecular Protozoology, Osaka University, Japan.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't