Linked Life Data

7641976

Source:http://linkedlifedata.com/resource/pubmed/id/7641976

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1995-9-21
pubmed:abstractText
The expression of the discoidin I genes in Dictyostelium discoideum is regulated by the concerted action of the extracellular factors cyclic adenosine monophosphate (cAMP), folate, prestarvation factor (PSF) and conditioned media factor (CMF). However, the pathways by which these signals are transduced and the interactions between the pathways have been unexplored so far. We have analysed wild-type and mutant cells with defined lesions in signal transduction to elucidate these regulatory processes, and shown that different pathways are used for the down-regulation and induction of these genes. The cAMP receptor cARI is required for the cAMP-mediated down-regulation of discoidin I gene expression but not for the induction of discoidin I expression during development. Surprisingly, induction of the discoidin I genes requires G alpha 2, the G-protein subunit which is generally believed to couple to cARI, to control the expression of cAMP-inducible genes. Thus, our data suggest that G alpha 2 interacts with different receptors to regulate gene expression in early development. Furthermore, the analysis shows that discoidin induction in bacterially grown cells occurs in two sequential steps. The first is a low basal induction which occurs in late log-phase growth prior to starvation. PSF can induce the basal level, and the induction is independent of G alpha 2. The developmental induction following starvation is much stronger, dependent on G alpha 2 and probably signaled by CMF, which is secreted at that time.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0301-4681
pubmed:author
pubmed:issnType
Print
pubmed:volume
58
pubmed:geneSymbol
G&agr;2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
253-60
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Multiple signal transduction pathways regulate discoidin I gene expression in Dictyostelium discoideum.
pubmed:affiliation
Max-Planck-Institut für Biochemie, Abteilung Zellbiologie, Martinsried, Germany.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't