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rdf:type |
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lifeskim:mentions |
umls-concept:C0017337,
umls-concept:C0025932,
umls-concept:C0027651,
umls-concept:C0033684,
umls-concept:C0086418,
umls-concept:C0086661,
umls-concept:C0678222,
umls-concept:C0679058,
umls-concept:C1414402,
umls-concept:C1415552,
umls-concept:C1518174,
umls-concept:C1547699,
umls-concept:C2700640
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pubmed:issue |
17
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pubmed:dateCreated |
1995-9-18
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pubmed:abstractText |
We have identified previously a gene from a human cervical carcinoma cell (HeLa) cDNA expression library that encodes a M(r) approximately 37,000 c-myc promoter-binding protein (MBP-1), which binds to the TATA box sequences of c-myc P2 promoter and exerts a negative regulatory function by down-regulating c-myc expression. In normal human tissues, this cloned gene showed variable expression. In this study, we have demonstrated that introduction of the MBP-1 gene into human breast carcinoma cells reduced their ability to invade through a basement membrane matrix in vitro but did not alter their growth rate. Human breast carcinoma transfected with MBP-1 cells showed a loss of anchorage-independent growth and also suppressed tumor formation in athymic nude mice. These results suggest the possible involvement of MBP-1 as a tumor suppressor gene in human breast carcinoma cells.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0008-5472
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
55
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pubmed:geneSymbol |
myc
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3747-51
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:7641187-Animals,
pubmed-meshheading:7641187-Base Sequence,
pubmed-meshheading:7641187-Breast Neoplasms,
pubmed-meshheading:7641187-Cell Adhesion,
pubmed-meshheading:7641187-Cell Division,
pubmed-meshheading:7641187-DNA-Binding Proteins,
pubmed-meshheading:7641187-Down-Regulation,
pubmed-meshheading:7641187-Humans,
pubmed-meshheading:7641187-Mice,
pubmed-meshheading:7641187-Molecular Sequence Data,
pubmed-meshheading:7641187-Neoplasm Invasiveness,
pubmed-meshheading:7641187-Neoplasm Proteins,
pubmed-meshheading:7641187-Phosphopyruvate Hydratase,
pubmed-meshheading:7641187-Proto-Oncogene Proteins c-myc,
pubmed-meshheading:7641187-Transfection,
pubmed-meshheading:7641187-Tumor Cells, Cultured,
pubmed-meshheading:7641187-Tumor Markers, Biological,
pubmed-meshheading:7641187-Tumor Suppressor Proteins
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pubmed:year |
1995
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pubmed:articleTitle |
Human breast carcinoma cells transfected with the gene encoding a c-myc promoter-binding protein (MBP-1) inhibits tumors in nude mice.
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pubmed:affiliation |
Department of Internal Medicine, Saint Louis University School of Medicine, Missouri 63110, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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