|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2-4
|
|
pubmed:dateCreated |
1995-9-14
|
|
pubmed:abstractText |
We report the identification of an Hb S homozygosity in a 29-year-old multiple-transfused patient with severe kidney disease using a new reverse-transcription polymerase chain reaction (RT-PCR) technique with mRNA as starting template. The procedure requires considerably less time than sequencing of genomic DNA and is recommended for use in the diagnosis of Hb S and other hemoglobinopathies.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:issn |
0001-5792
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
93
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
105-7
|
|
pubmed:dateRevised |
2007-11-14
|
|
pubmed:meshHeading |
pubmed-meshheading:7639050-Adult,
pubmed-meshheading:7639050-Anemia, Sickle Cell,
pubmed-meshheading:7639050-Base Sequence,
pubmed-meshheading:7639050-Blood Transfusion,
pubmed-meshheading:7639050-Deoxyribonucleases, Type II Site-Specific,
pubmed-meshheading:7639050-Female,
pubmed-meshheading:7639050-Globins,
pubmed-meshheading:7639050-Glomerulosclerosis, Focal Segmental,
pubmed-meshheading:7639050-Hemoglobin, Sickle,
pubmed-meshheading:7639050-Humans,
pubmed-meshheading:7639050-Kidney Transplantation,
pubmed-meshheading:7639050-Molecular Sequence Data,
pubmed-meshheading:7639050-Polymerase Chain Reaction,
pubmed-meshheading:7639050-Polymorphism, Restriction Fragment Length,
pubmed-meshheading:7639050-RNA, Messenger,
pubmed-meshheading:7639050-Renal Dialysis
|
|
pubmed:year |
1995
|
|
pubmed:articleTitle |
Sickle cell anemia identified in a multiple-transfused patient through analysis of mRNA with an RT-PCR-based technique.
|
|
pubmed:affiliation |
Department of Biochemistry, Medical College of Georgia, Augusta 30912-2100, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Case Reports
|
