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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
16
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pubmed:dateCreated |
1995-9-11
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pubmed:abstractText |
The 5' flanking region of the human alpha-globin gene is highly G + C rich and contains multiple copies of the consensus sequence for the Sp1 binding site. We investigated the role of this G + C-rich region in augmenting alpha-globin promoter activity in the presence of the far-upstream alpha-globin enhancer, HS-40. We show that in transiently transfected erythroid cells, deletion of the alpha-globin G + C-rich 5' flanking region has no effect on alpha-globin promoter activity. However, upon stable integration into chromatin, deletion of this region causes a nearly 90% decrease in promoter activity compared with expression from an alpha-globin promoter retaining this region. These results suggest that the alpha-globin G + C-rich 5' flanking region augments alpha-globin promoter activity in a chromatin-dependent manner. We further show that this G + C-rich region is required for the activation of alpha-globin gene expression during erythroid differentiation. Finally, we show by both footprint analysis and functional assays that the ability of the G + C-rich region to increase alpha-globin promoter activity from a stably integrated alpha-globin gene is mediated by its multiple binding sites for the transcription factor Sp1.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7638173-1454528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7638173-1535687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7638173-1591777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7638173-1741249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7638173-1875946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7638173-2253879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7638173-2317863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7638173-2538719,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7638173-2649166,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7638173-2704733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7638173-2748594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7638173-2922063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7638173-2992804,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7638173-2996137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7638173-3349524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7638173-3542191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7638173-354501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7638173-3595568,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7638173-3690667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7638173-3879975,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7638173-3905996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7638173-6828386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7638173-6960240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7638173-7532132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7638173-8248238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7638173-8428926,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7638173-8503862
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
92
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7237-41
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7638173-Animals,
pubmed-meshheading:7638173-Base Composition,
pubmed-meshheading:7638173-Base Sequence,
pubmed-meshheading:7638173-Binding Sites,
pubmed-meshheading:7638173-Cell Line,
pubmed-meshheading:7638173-Chromatin,
pubmed-meshheading:7638173-Consensus Sequence,
pubmed-meshheading:7638173-Enhancer Elements, Genetic,
pubmed-meshheading:7638173-Erythrocytes,
pubmed-meshheading:7638173-Erythropoiesis,
pubmed-meshheading:7638173-Globins,
pubmed-meshheading:7638173-Humans,
pubmed-meshheading:7638173-Mice,
pubmed-meshheading:7638173-Molecular Sequence Data,
pubmed-meshheading:7638173-Plasmids,
pubmed-meshheading:7638173-Promoter Regions, Genetic,
pubmed-meshheading:7638173-Sp1 Transcription Factor,
pubmed-meshheading:7638173-Transfection
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pubmed:year |
1995
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pubmed:articleTitle |
Sp1 functions in a chromatin-dependent manner to augment human alpha-globin promoter activity.
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pubmed:affiliation |
Sir William Dunn School of Pathology, Chemical Pathology Unit, Oxford University, United Kingdom.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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