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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
16
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pubmed:dateCreated |
1995-9-11
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pubmed:abstractText |
Serotonergic agents (uptake inhibitors, receptor ligands) cause significant craniofacial malformations in cultured mouse embryos suggesting that 5-hydroxytryptamine (serotonin) (5-HT) may be an important regulator of craniofacial development. To determine whether serotonergic regulation of cell migration might underly some of these effects, cranial neural crest (NC) explants from embryonic day 9 (E9) (plug day = E1) mouse embryos or dissociated mandibular mesenchyme cells (derived from NC) from E12 embryos were placed in a modified Boyden chamber to measure effects of serotonergic agents on cell migration. A dose-dependent effect of 5-HT on the migration of highly motile cranial NC cells was demonstrated, such that low concentrations of 5-HT stimulated migration, whereas this effect was progressively lost as the dose of 5-HT was increased. In contrast, most concentrations of 5-HT inhibited migration of less motile, mandibular mesenchyme cells. To investigate the possible involvement of specific 5-HT receptors in the stimulation of NC migration, several 5-HT subtype-selective antagonists were used to block the effects of the most stimulatory dose of 5-HT (0.01 microM). Only NAN-190 (a 5-HT1A antagonist) inhibited the effect of 5-HT, suggesting involvement of this receptor. Further evidence was obtained by using immunohistochemistry with 5-HT receptor antibodies, which revealed expression of the 5-HT1A receptor but not other subtypes by migrating NC cells in both embryos and cranial NC explants. These results suggest that by activating appropriate receptors 5-HT may regulate migration of cranial NC cells and their mesenchymal derivatives in the mouse embryo.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7638165-1283734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7638165-1323420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7638165-13320119,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7638165-1412065,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7638165-14251469,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/7638165-8517851
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
92
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7182-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7638165-Animals,
pubmed-meshheading:7638165-Cell Movement,
pubmed-meshheading:7638165-Dose-Response Relationship, Drug,
pubmed-meshheading:7638165-Female,
pubmed-meshheading:7638165-Immunohistochemistry,
pubmed-meshheading:7638165-Mice,
pubmed-meshheading:7638165-Mice, Inbred ICR,
pubmed-meshheading:7638165-Neural Crest,
pubmed-meshheading:7638165-Piperazines,
pubmed-meshheading:7638165-Pregnancy,
pubmed-meshheading:7638165-Receptors, Serotonin,
pubmed-meshheading:7638165-Serotonin,
pubmed-meshheading:7638165-Serotonin Antagonists
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pubmed:year |
1995
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pubmed:articleTitle |
Serotonin regulates mouse cranial neural crest migration.
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pubmed:affiliation |
Department of Cell Biology and Anatomy, School of Medicine, University of North Carolina, Chapel Hill 27599-7090, USA.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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