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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1995-9-11
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pubmed:abstractText |
Apo E, a key regulator of cholesterol-rich lipoprotein metabolism, is synthesized by numerous extrahepatic tissues. Although its synthesis in macrophages is documented, the contribution of macrophage-derived apo E to hepatic clearance of serum cholesterol is unknown. To address this issue bone marrow transplantation was performed on hypercholesterolemic apo E-deficient mice with either syngeneic apo E-deficient mouse bone marrow cells (E0-control) or wild-type mouse bone marrow cells expressing apo E (E0-treated). E0-control and E0-treated mice were fed either a regular chow diet or an atherogenic diet (designated E0-control-HF and E0-treated-HF). Serum cholesterol levels dropped dramatically in the E0-treated mice largely due to a reduction in their VLDL cholesterol. No changes were seen in the E0-control mice. After 4 wk serum cholesterol in E0-treated-HF mice was about four-fold lower compared to E0-control-HF animals. Moreover, the extent of atherosclerosis in the E0-treated-HF mice after 14-16 wk was greatly reduced. Wild-type apo E mRNA was detected in the liver, spleen, and brain of the E0-treated mice indicating that apo E gene transfer was successfully achieved through bone marrow transplantation. More importantly, the level of apo E expression was sufficient to reduce the severe hypercholesterolemia of the apo E-deficient mice fed either chow or atherogenic diets.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635947-1411543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635947-1423598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635947-1584779,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635947-2056122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635947-2440339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635947-2644082,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635947-2723547,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635947-2779654,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635947-2995353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635947-3531238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635947-356266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635947-3882695,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635947-3931677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635947-3973536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635947-4384568,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635947-4552638,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635947-4559194,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635947-4986254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635947-5655103,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635947-6233768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635947-6286633,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635947-6343371,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635947-6530593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635947-6950395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635947-7511933,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635947-775638,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635947-7944147
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0021-9738
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
96
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1118-24
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7635947-Alleles,
pubmed-meshheading:7635947-Animals,
pubmed-meshheading:7635947-Apolipoproteins E,
pubmed-meshheading:7635947-Arteriosclerosis,
pubmed-meshheading:7635947-Base Sequence,
pubmed-meshheading:7635947-Bone Marrow Transplantation,
pubmed-meshheading:7635947-Cholesterol,
pubmed-meshheading:7635947-Diet, Atherogenic,
pubmed-meshheading:7635947-Gene Therapy,
pubmed-meshheading:7635947-Gene Transfer Techniques,
pubmed-meshheading:7635947-Hyperlipoproteinemia Type II,
pubmed-meshheading:7635947-Macrophages,
pubmed-meshheading:7635947-Mice,
pubmed-meshheading:7635947-Mice, Inbred C57BL,
pubmed-meshheading:7635947-Molecular Sequence Data,
pubmed-meshheading:7635947-Organ Specificity,
pubmed-meshheading:7635947-Polymerase Chain Reaction,
pubmed-meshheading:7635947-RNA, Messenger
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pubmed:year |
1995
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pubmed:articleTitle |
Treatment of severe hypercholesterolemia in apolipoprotein E-deficient mice by bone marrow transplantation.
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pubmed:affiliation |
Department of Immunology, Scripps Research Institute, La Jolla, California 92037, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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