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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1995-9-13
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pubmed:abstractText |
Double-strand DNA breaks (DSBs) initiate meiotic recombination in Saccharomyces cerevisiae. DSBs occur at sites that are hypersensitive in nuclease digests of chromatin, suggesting a role for chromatin structure in determining DSB location. We show here that the frequency of DSBs at a site is not determined simply by DNA sequence or by features of chromatin structure. An arg4-containing plasmid was inserted at several different locations in the yeast genome. Meiosis-induced DSBs occurred at similar sites in pBR322-derived portions of the construct at all insert loci, and the frequency of these breaks varied in a manner that mirrored the frequency of meiotic recombination in the arg4 portion of the insert. However, DSBs did not occur in the insert-borne arg4 gene at a site that is frequently broken at the normal ARG4 locus, even though the insert-borne arg4 gene and the normal ARG4 locus displayed similar DNase I hypersensitivity patterns. Deletions that removed active DSB sites from an insert at HIS4 restored breaks to the insert-borne arg4 gene and to a DSB site in flanking chromosomal sequences. We conclude that the frequency of DSB at a site can be affected by sequences several thousand nucleotides away and suggest that this is because of competition between DSB sites for locally limited factors.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635308-1304426,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635308-1324174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635308-1465441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635308-1480108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635308-1535156,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635308-1570334,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635308-1574125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635308-1781031,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635308-1819520,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635308-1913808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635308-2004421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635308-2087779,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635308-2185891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635308-2190690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635308-2537472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635308-2645528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635308-3058548,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635308-388424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635308-3909105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635308-4595206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635308-6276023,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635308-6330094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635308-6394957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635308-7035683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635308-7862159,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635308-7988571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635308-7991559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635308-7991560,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635308-8020104,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635308-8290959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635308-8319906,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635308-8417336,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635308-8431941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635308-8436266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635308-8455614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7635308-8467798
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0016-6731
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
140
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pubmed:geneSymbol |
ARG4,
HIS4,
LEU2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
55-66
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7635308-Argininosuccinate Lyase,
pubmed-meshheading:7635308-Chromatin,
pubmed-meshheading:7635308-Crossing Over, Genetic,
pubmed-meshheading:7635308-DNA,
pubmed-meshheading:7635308-DNA, Fungal,
pubmed-meshheading:7635308-DNA Damage,
pubmed-meshheading:7635308-Fungal Proteins,
pubmed-meshheading:7635308-Meiosis,
pubmed-meshheading:7635308-Recombination, Genetic,
pubmed-meshheading:7635308-Saccharomyces cerevisiae,
pubmed-meshheading:7635308-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:7635308-Sequence Deletion
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pubmed:year |
1995
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pubmed:articleTitle |
Factors that affect the location and frequency of meiosis-induced double-strand breaks in Saccharomyces cerevisiae.
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pubmed:affiliation |
Laboratory of Biochemistry, National Cancer Institute, Bethesda, Maryland 20892, USA.
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pubmed:publicationType |
Journal Article
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