Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1995-9-14
|
| pubmed:abstractText |
We have applied two-colour fluorescence in situ hybridization (FISH) to DNA fibers and combined it with digital imaging microscopy for the mapping of large cosmid contigs. The technique was validated using a set of unique plasmids and a cosmid contig both originating from the thyroglobulin (Tg) gene and previously mapped by restriction analysis. The resolution proved to be close to the theoretical lower limit of approximately 1 kb, ranging > or = 400 kb. Subsequently a 400 kb cosmid contig derived from a DMD-YAC was directly mapped by Fiber-FISH. The resulting map is in full agreement with the restriction map. Two-colour Fiber-FISH mapping thus showed to be capable for accurately sizing gaps and overlaps, and to identify chimeric or repeat sequence containing cosmids across a 400 kb region at once. The generated 400 kb 'colour bar-code' was subsequently used to map two DMD deletion breakpoints in patient DNA with an accuracy of 1-2 kb. The results underscore the value of this method for the delineation of chromosomal rearrangements for positional cloning and single patient clinical studies.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0964-6906
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
4
|
| pubmed:geneSymbol |
DMD
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
831-6
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7633442-Chimera,
pubmed-meshheading:7633442-Chromosome Mapping,
pubmed-meshheading:7633442-Chromosomes, Artificial, Yeast,
pubmed-meshheading:7633442-Cloning, Molecular,
pubmed-meshheading:7633442-Color,
pubmed-meshheading:7633442-Cosmids,
pubmed-meshheading:7633442-DNA,
pubmed-meshheading:7633442-Gene Deletion,
pubmed-meshheading:7633442-Gene Rearrangement,
pubmed-meshheading:7633442-Genome, Human,
pubmed-meshheading:7633442-Humans,
pubmed-meshheading:7633442-Image Processing, Computer-Assisted,
pubmed-meshheading:7633442-In Situ Hybridization, Fluorescence,
pubmed-meshheading:7633442-Male,
pubmed-meshheading:7633442-Muscular Dystrophies,
pubmed-meshheading:7633442-Plasmids,
pubmed-meshheading:7633442-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:7633442-Restriction Mapping,
pubmed-meshheading:7633442-Thyroglobulin
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
High-resolution DNA Fiber-FISH for genomic DNA mapping and colour bar-coding of large genes.
|
| pubmed:affiliation |
Department of Cytochemistry and Cytometry, State University Leiden, The Netherlands.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|