Linked Life Data

7632830

Source:http://linkedlifedata.com/resource/pubmed/id/7632830

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1995-9-13
pubmed:abstractText
It has been shown that the cAMP concentrations in capacitated mouse sperm can be increased in response to the egg's extracellular matrix, the zona pellucida (ZP), during the acrosome reaction. However, it is not known whether this cAMP elevation is caused by a stimulation of adenylyl cyclase (AC) activity or a decrease in cyclic nucleotide phosphodiesterase activity. In the present study using capacitated mouse spermatozoa, 20% and 30% of the total AC activity in the presence of Mg2+ and Mn2+, respectively, was measured in the sperm membrane fraction that contained the highest specific AC activity. As reported in other studies of sperm AC, Mn(2+)-supported AC activity was 15-30 times higher than Mg(2+)-supported AC activity. Interestingly, when the membrane fraction was solubilized with Lubrol-PX, an increase in specific and total Mg(2+)-supported AC activity was obtained while a decrease in both the specific and total Mn(2+)-supported AC activity was observed. In the absence of detergent, the ZP stimulates the membrane AC in a concentration-dependent manner, but this stimulation is observed only when the enzyme activity is measured in the presence of Mg2+. Forskolin also stimulates the Mg(2+)-but not the Mn(2+)-supported AC activity, and this effects is not additive to or synergistic with the ZP stimulation of the membrane AC. Results reported in this study suggest that ZP-mediated stimulation of membrane-associated AC may represent a mechanism by which the ZP affects sperm functions.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0006-3363
pubmed:author
pubmed:issnType
Print
pubmed:volume
52
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1227-33
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Mouse sperm adenylyl cyclase: general properties and regulation by the zona pellucida.
pubmed:affiliation
Department of Obstetrics and Gynecology, University of Pennsylvania School of Medicine, Philadelphia 19104-6080, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't