Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1995-9-5
|
| pubmed:abstractText |
A simple method of visual membrane enzyme-immunoassay (EIA) for the detection of progesterone is described. When two types of progesterone-horseradish peroxidase (HRP) tracers were challenged for binding, in the presence of progesterone, to the monoclonal anti-progesterone antibody, 15A, coated on the microtitre plate, the HRP conjugated at the C-3 position (A-ring) of progesterone competed more effectively with progesterone to the binding site of the monoclonal antibody (mAb) than HRP conjugated at the C-11 position of the C-ring. By using this combination of mAb, 15A, and progesterone-3(O-carboxymethyloxime)-HRP (P-3CMO-HRP), we developed a visual membrane EIA system in which free progesterone in the sample could be quantified by the degree of color development. In this system, free progesterone competed with P-3CMO-HRP for binding sites of mAb immobilized on the nitrocellulose membrane. The stable grey color was formed on the surface of membrane for progesterone-negative and no color for progesterone-positive sample using 3,3'-diaminobenzidine (DAB) with Co2+ as an insoluble substrate solution. To examine whether tetramethylbenzidine (TMB) can substitute for DAB in membrane EIA, an experiment was conducted where TMB was used as an insoluble substrate.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/3,3',5,5'-tetramethylbenzidine,
http://linkedlifedata.com/resource/pubmed/chemical/3,3'-Diaminobenzidine,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Benzidines,
http://linkedlifedata.com/resource/pubmed/chemical/Cations, Divalent,
http://linkedlifedata.com/resource/pubmed/chemical/Chromogenic Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Cobalt,
http://linkedlifedata.com/resource/pubmed/chemical/Collodion,
http://linkedlifedata.com/resource/pubmed/chemical/Horseradish Peroxidase,
http://linkedlifedata.com/resource/pubmed/chemical/Membranes, Artificial,
http://linkedlifedata.com/resource/pubmed/chemical/Progesterone,
http://linkedlifedata.com/resource/pubmed/chemical/progesterone-3-(O-carboxymethyl...
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0197-1522
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
16
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
137-53
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7629275-3,3'-Diaminobenzidine,
pubmed-meshheading:7629275-Antibodies, Monoclonal,
pubmed-meshheading:7629275-Antigen-Antibody Reactions,
pubmed-meshheading:7629275-Benzidines,
pubmed-meshheading:7629275-Binding, Competitive,
pubmed-meshheading:7629275-Cations, Divalent,
pubmed-meshheading:7629275-Chromogenic Compounds,
pubmed-meshheading:7629275-Cobalt,
pubmed-meshheading:7629275-Collodion,
pubmed-meshheading:7629275-Horseradish Peroxidase,
pubmed-meshheading:7629275-Immunoenzyme Techniques,
pubmed-meshheading:7629275-Membranes, Artificial,
pubmed-meshheading:7629275-Progesterone
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Use of progesterone-3(O-carboxymethyl oxime)-horseradish peroxidase in a sensitive microtitre-plate EIA and its application to a visual membrane EIA of progesterone.
|
| pubmed:affiliation |
Genetic Engineering Research Institute, Korea Institute of Science and Technology (KIST), Taejon.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|