Linked Life Data

7628696

Source:http://linkedlifedata.com/resource/pubmed/id/7628696

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
13
pubmed:dateCreated
1995-9-1
pubmed:abstractText
Extremes of pH are an occupational hazard for many microorganisms. In addition to efficient pH homeostasis, survival effectively requires a regulatory system tailoring the syntheses of molecules functioning beyond the cell boundaries (permeases, secreted enzymes, and exported metabolites) to the pH of the growth environment. Our previous work established that the zinc finger PacC transcription factor mediates such pH regulation in the fungus Aspergillus nidulans in response to a signal provided by the products of the six pal genes at alkaline ambient pH. In the presence of this signal, PacC becomes functional, activating transcription of genes expressed at alkaline pH and preventing transcription of genes expressed at acidic pH. Here we detect two forms of PacC in extracts, both forming specific retardation complexes with a PacC-binding site. Under acidic growth conditions or in acidity-mimicking pal mutants (defective in ambient pH signal transduction), the full-length form of PacC predominates. Under alkaline growth conditions or in alkalinity-mimicking pacCc mutants (independent of the ambient pH signal), a proteolysed version containing the amino-terminal approximately 40% of the protein predominates. This specifically cleaved shorter version is clearly functional, both as an activator for alkaline-expressed genes and as a repressor for acid-expressed genes, but the full-length form of PacC must be inactive. Thus, PacC proteolysis is an essential and pH-sensitive step in the regulation of gene expression by ambient pH. Carboxy-terminal truncations, resulting in a gain-of-function (pacCc) phenotype, bypass the requirement for the pal signal transduction pathway for conversion of the full-length to the proteolyzed functional form.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0890-9369
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
9
pubmed:geneSymbol
pacC, pacC<up>c</up>
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1622-32
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:7628696-Aspergillus nidulans, pubmed-meshheading:7628696-Base Sequence, pubmed-meshheading:7628696-Binding Sites, pubmed-meshheading:7628696-Blotting, Western, pubmed-meshheading:7628696-Consensus Sequence, pubmed-meshheading:7628696-Endopeptidases, pubmed-meshheading:7628696-Fungal Proteins, pubmed-meshheading:7628696-Gene Expression Regulation, Fungal, pubmed-meshheading:7628696-Genes, Fungal, pubmed-meshheading:7628696-Genotype, pubmed-meshheading:7628696-Hydrogen-Ion Concentration, pubmed-meshheading:7628696-Mutagenesis, Site-Directed, pubmed-meshheading:7628696-Protein Biosynthesis, pubmed-meshheading:7628696-Recombinant Fusion Proteins, pubmed-meshheading:7628696-Transcription, Genetic, pubmed-meshheading:7628696-Transcription Factors, pubmed-meshheading:7628696-Zinc Fingers
pubmed:year
1995
pubmed:articleTitle
Activation of the Aspergillus PacC transcription factor in response to alkaline ambient pH requires proteolysis of the carboxy-terminal moiety.
pubmed:affiliation
Departamento de Microbiología Molecular, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't