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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1995-9-7
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pubmed:abstractText |
Several deletion mutants of Raf-1 were expressed with v-ras p21 or 14-3-3 protein in COS-7 cells and Sf9 cells and the interaction of Raf-1 with ras p21 or with 14-3-3 protein in intact cells was examined. Raf(1-135) (residues 1-135) and Raf(1-322) interacted with v-ras p21, but other deletion mutants such as Raf(136-322) or Raf(321-648) did not. Raf(1-322) interacted with 14-3-3 protein much more efficiently than Raf(321-648) did. While Raf(1-135) did not interact with 14-3-3 protein, Raf(136-322) did. These results clearly indicate that Raf-1 simultaneously interacts with both ras p21 and 14-3-3 protein through the distinct binding domains in intact cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
17
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pubmed:volume |
368
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
321-5
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7628630-14-3-3 Proteins,
pubmed-meshheading:7628630-Animals,
pubmed-meshheading:7628630-Binding Sites,
pubmed-meshheading:7628630-Cell Line,
pubmed-meshheading:7628630-Haplorhini,
pubmed-meshheading:7628630-Humans,
pubmed-meshheading:7628630-Oncogene Protein p21(ras),
pubmed-meshheading:7628630-Protein-Serine-Threonine Kinases,
pubmed-meshheading:7628630-Proteins,
pubmed-meshheading:7628630-Proto-Oncogene Proteins,
pubmed-meshheading:7628630-Proto-Oncogene Proteins c-raf,
pubmed-meshheading:7628630-Sequence Deletion,
pubmed-meshheading:7628630-Spodoptera,
pubmed-meshheading:7628630-Tyrosine 3-Monooxygenase
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pubmed:year |
1995
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pubmed:articleTitle |
Characterization of the interaction of Raf-1 with ras p21 or 14-3-3 protein in intact cells.
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pubmed:affiliation |
Department of Biochemistry, Hiroshima University School of Medicine, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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