Linked Life Data

7628404

Source:http://linkedlifedata.com/resource/pubmed/id/7628404

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1995-9-1
pubmed:abstractText
Previous studies from our laboratory established that large M(r) mucin glycoproteins are major apically disposed components of mouse uterine epithelial cells in vitro. The present studies demonstrate that Muc-1 represents one of the apically disposed mucin glycoproteins of mouse uterine epithelia, and that Muc-1 protein and messenger RNA (mRNA) expression are regulated in the periimplantation mouse uterus by ovarian steroids. Muc-1 expression is exclusive to the epithelial cells of the uterus under all conditions examined. Muc-1 expression is high in the proestrous and estrous stages and decreases during diestrous. Both Muc-1 protein and mRNA decline to barely detectable levels by day 4 of pregnancy, i.e. before the time of blastocyst attachment. In contrast, Muc-1 expression in the cervix and vagina is maintained during this same period. Delayed implantation was established in pregnant mice by ovariectomy and maintained by the administration of exogenous progesterone (P). Initiation of implantation was triggered by coinjection of P-maintained mice with a nidatory dose of 17 beta-estradiol (E2). Muc-1 levels in the uterine epithelia of P-maintained mice declined to low levels similar to those observed on day 4 of normal pregnancy. Coinjection of E2 did not alter Muc-1 expression, suggesting that down-regulation of Muc-1 is a P-dominated event. This was confirmed in ovariectomized nonpregnant mice, which displayed stimulation of Muc-1 expression after 6 h of E2 injection. E2-Stimulated Muc-1 expression was inhibited by the pure antiestrogen, ICI 164,384. Although P alone had no effect on Muc-1 expression, it antagonized the action of E2. Injection of pregnant mice with the antiprogestin, RU486, a known implantation inhibitor, on day 3 of pregnancy restored high level expression of Muc-1 mRNA on day 4, indicating that down-regulation of Muc-1 is P receptor mediated. Collectively, these data indicate that Muc-1 expression in mouse uterine epithelium is strongly influenced by ovarian steroids. It is suggested that the loss of Muc-1 contributes to generation of a receptive uterine state.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0013-7227
pubmed:author
pubmed:issnType
Print
pubmed:volume
136
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3639-47
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:7628404-Animals, pubmed-meshheading:7628404-Binding Sites, pubmed-meshheading:7628404-Embryo Implantation, pubmed-meshheading:7628404-Estradiol, pubmed-meshheading:7628404-Estrus, pubmed-meshheading:7628404-Female, pubmed-meshheading:7628404-Genitalia, Female, pubmed-meshheading:7628404-Immune Sera, pubmed-meshheading:7628404-Membrane Glycoproteins, pubmed-meshheading:7628404-Mice, pubmed-meshheading:7628404-Mice, Inbred Strains, pubmed-meshheading:7628404-Mifepristone, pubmed-meshheading:7628404-Mucin-1, pubmed-meshheading:7628404-Mucins, pubmed-meshheading:7628404-Precipitin Tests, pubmed-meshheading:7628404-Pregnancy, pubmed-meshheading:7628404-Progesterone, pubmed-meshheading:7628404-RNA, Messenger, pubmed-meshheading:7628404-Uterus, pubmed-meshheading:7628404-Wheat Germ Agglutinins
pubmed:year
1995
pubmed:articleTitle
Expression and steroid hormonal control of Muc-1 in the mouse uterus.
pubmed:affiliation
Department of Biochemistry and Molecular Biology, M. D. Anderson Cancer Center, Houston, Texas 77030, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.