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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1995-8-25
|
| pubmed:databankReference | |
| pubmed:abstractText |
A polymerase chain reaction-generated mouse ACTH-R probe was used to screen a mouse genomic library, and a clone of 13kb containing the entire coding sequence was isolated. The coding sequence shows 84% homology with the human gene at the DNA level and encodes a peptide with 89% homology to the human ACTH-R. This gene is expressed as a major transcript of 1.8kb in the mouse adrenal gland. The gene was expressed in HeLa cells and cAMP production in response to either ACTH or alpha-MSH was measured. cAMP increased in an ACTH dose dependent manner suggesting an EC50 of 7 x 10(-10)M ACTH. alpha-MSH was without effect on this receptor. In conclusion we have cloned a mouse ACTH receptor gene and demonstrated for the first time its expression and functional effect in HeLa cells.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0006-291X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
26
|
| pubmed:volume |
212
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
912-8
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7626130-Amino Acid Sequence,
pubmed-meshheading:7626130-Animals,
pubmed-meshheading:7626130-Base Sequence,
pubmed-meshheading:7626130-Cattle,
pubmed-meshheading:7626130-Cloning, Molecular,
pubmed-meshheading:7626130-Cyclic AMP,
pubmed-meshheading:7626130-DNA,
pubmed-meshheading:7626130-Gene Expression,
pubmed-meshheading:7626130-Genomic Library,
pubmed-meshheading:7626130-HeLa Cells,
pubmed-meshheading:7626130-Humans,
pubmed-meshheading:7626130-Mice,
pubmed-meshheading:7626130-Molecular Sequence Data,
pubmed-meshheading:7626130-Polymerase Chain Reaction,
pubmed-meshheading:7626130-Receptors, Corticotropin,
pubmed-meshheading:7626130-Sequence Homology, Amino Acid,
pubmed-meshheading:7626130-Species Specificity,
pubmed-meshheading:7626130-Transfection
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Cloning, characterization and expression of a functional mouse ACTH receptor.
|
| pubmed:affiliation |
Department of Chemical Endocrinology, St Bartholomew's Hospital Medical College, London, United Kingdom.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|