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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1995-8-25
|
| pubmed:databankReference | |
| pubmed:abstractText |
ATP-regulated potassium channels play a key role in regulating insulin secretion. We have isolated cDNA clones from a RINm5F insulinoma cell cDNA library that encode a protein, KATP-2, whose sequence shows 72% identity with the rat heart potassium channel KATP. RNA blotting showed that KATP-2 mRNA was present at high levels in brain and undetectable in heart, spleen, lung, liver, skeletal muscle, kidney and testis. A quantitative RT-PCR assay indicated that there were 1.85 +/- 0.32 x 10(5) molecules of KATP-2 mRNA per microgram of total RNA in pancreatic islets from nondiabetic rats. The levels of KATP-2 mRNA were reduced by 34% in islets from diabetic Zucker diabetic fatty male rats, a model of non-insulin dependent diabetes mellitus, compared to their lean nondiabetic littermates (p < 0.05), suggesting that decreased expression of KATP-2 may contribute to beta-cell dysfunction in this animal model.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0006-291X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
26
|
| pubmed:volume |
212
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
894-9
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:7626127-Adenosine Triphosphate,
pubmed-meshheading:7626127-Amino Acid Sequence,
pubmed-meshheading:7626127-Animals,
pubmed-meshheading:7626127-Base Sequence,
pubmed-meshheading:7626127-Cloning, Molecular,
pubmed-meshheading:7626127-DNA Primers,
pubmed-meshheading:7626127-Diabetes Mellitus, Experimental,
pubmed-meshheading:7626127-Islets of Langerhans,
pubmed-meshheading:7626127-Male,
pubmed-meshheading:7626127-Molecular Sequence Data,
pubmed-meshheading:7626127-Obesity,
pubmed-meshheading:7626127-Polymerase Chain Reaction,
pubmed-meshheading:7626127-Potassium Channels,
pubmed-meshheading:7626127-RNA, Messenger,
pubmed-meshheading:7626127-Rats,
pubmed-meshheading:7626127-Rats, Zucker,
pubmed-meshheading:7626127-Sequence Homology, Amino Acid,
pubmed-meshheading:7626127-Tissue Distribution
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Cloning of rat KATP-2 channel and decreased expression in pancreatic islets of male Zucker diabetic fatty rats.
|
| pubmed:affiliation |
Howard Hughes Medical Institute, University of Chicago, Illinois 60637, USA.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|