Linked Life Data

7626025

Source:http://linkedlifedata.com/resource/pubmed/id/7626025

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1995-8-29
pubmed:abstractText
Sixteen isolates of thermophilic bacteria from the genus Thermus, isolated from neutral and alkaline hot water springs in the southwest region of Iceland, were tested for the presence of restriction endonucleases. Extracts from five of the isolates showed evidence of the presence of restriction endonuclease activity by producing discrete nucleotide fragments when incubated at 65 degrees C with lambda phage DNA. Two of the isolates (Tsp4C and Tsp8E) were found to have particularly high levels of restriction endonuclease activity, and the respective enzymes from these two Thermus isolates were partially purified and characterized and their recognition and cleavage sites were determined. Enzyme Tsp4C I is a novel Type II restriction endonuclease recognizing the interrupted palindromic tetranucleotide sequence ACNGT, where N can be any one of the four bases in DNA. Tsp4C I, which retains full enzyme activity when incubated for 10 min at temperatures up to 76 degrees C, hydrolyses the phosphodiester bond in both strands of a double-stranded DNA substrate between the third and fourth bases of the recognition sequence (ACN/GT), generating fragments with a single base 3'-OH overhang. Enzyme Tsp8E I is a thermostable isoschizomer of the mesophilic Type II restriction endonuclease Bgl I (GCCNNNN/NGGC) [Lee, Clanton and Chirikjiam (1979) Fed. Proc. 28, 294], generating fragments with a three base 3'-OH overhang. However, unlike Bgl I, Tsp8E I exhibits considerable thermal stability, retaining full enzyme activity when incubated for 10 min at temperatures up to 78 degrees C. Both Tsp4C I and Tsp8E I represent significant additions to the small but expanding list of the extremely thermostable restriction endonucleases.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7626025-1097586, http://linkedlifedata.com/resource/pubmed/commentcorrection/7626025-1986365, http://linkedlifedata.com/resource/pubmed/commentcorrection/7626025-3806053, http://linkedlifedata.com/resource/pubmed/commentcorrection/7626025-457653, http://linkedlifedata.com/resource/pubmed/commentcorrection/7626025-4868368, http://linkedlifedata.com/resource/pubmed/commentcorrection/7626025-5432016, http://linkedlifedata.com/resource/pubmed/commentcorrection/7626025-5781580, http://linkedlifedata.com/resource/pubmed/commentcorrection/7626025-6246360, http://linkedlifedata.com/resource/pubmed/commentcorrection/7626025-6253357, http://linkedlifedata.com/resource/pubmed/commentcorrection/7626025-678024, http://linkedlifedata.com/resource/pubmed/commentcorrection/7626025-8392714
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
309 ( Pt 2)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
595-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Two thermostable type II restriction endonucleases from Icelandic strains of the genus Thermus: Tsp4C I (ACN/GT), a novel type II restriction endonuclease, and Tsp8E I, an isoschizomer of the mesophilic enzyme Bgl I (GCCNNNN/NGGC).
pubmed:affiliation
Department of Biochemistry, Faculty of Basic Medical Sciences, Queen Mary and Westfield College, University of London, U.K.
pubmed:publicationType
Journal Article