Linked Life Data

7624948

Source:http://linkedlifedata.com/resource/pubmed/id/7624948

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1995-8-31
pubmed:abstractText
It has been more difficult to prevent intestinal allograft rejection than rejection of other solid organ transplants. Epithelial cells in the small intestine have the potential to actively participate in graft rejection because they constitutively express MHC class II antigens. The present study examined the ability of intestinal epithelial cells (IEC) to stimulate allogeneic T cells in vitro using one-way MLC. IEC from jejunum and ileum of ACI rats (RT1a) were used as the stimulator cells; purified T lymphocytes from Lewis rats (RT11) were used as the responder cells. Freshly isolated IEC were poor stimulators of naive allogeneic T lymphocytes. However, IEC induced a strong proliferation of primed allogeneic T lymphocytes, and this response could be blocked by anti-MHC class II mAb (OX-4). IEC stimulated a vigorous proliferation of naive T cells when co-cultured with (1) macrophages syngeneic with responder T lymphocytes, (2) supernatants of LPS-stimulated macrophages, or (3) a combination of IL-1 and IL-6. We conclude that MHC class II positive IEC require soluble costimulatory factors to fully activate naive allogeneic T cells. Immune stimulation by enterocytes may be one of the reasons why it is so difficult to prevent intestinal allograft rejection. Strategies that block interactions among IEC, macrophages, and lymphocytes may reduce the immunogenicity of small bowel transplants.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0041-1337
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
60
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
82-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Allogeneic lymphocyte proliferation stimulated by small intestine-derived epithelial cells.
pubmed:affiliation
Department of Surgery, University of Western Ontario, London, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't