Linked Life Data

7624887

Source:http://linkedlifedata.com/resource/pubmed/id/7624887

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1995-8-29
pubmed:abstractText
Genotoxicity and cytotoxicity were evaluated in rainbow trout hepatocytes exposed to sediment extracts obtained in the vicinity of a creosote-treated wharf. Sediment cores were collected at the intertidal and subtidal sections of the wharf at distances of 1, 5, 40 and 50 m. Moreover, subsamples were also taken at different depths of the cores ranging from 2 to 10 cm below the sediment/water interface. Sediment samples were air-dried and extracted in dichloromethane followed with an exchange into dimethylsulfoxide (DMSO). Rainbow trout hepatocytes were exposed for 24 h at 15 degrees C to several concentrations of the sediment extract. Afterwards, the cells were collected, and cell viability was assayed along with genotoxicity using the nick translation and the alkaline precipitation assays. Results showed that the wharf contained high concentration of polycyclic aromatic hydrocarbons (PAHs), displayed genotoxicity and cytotoxicity to hepatocytes. In addition, PAHs, cytotoxicity and sometimes genotoxicity were detected in all sediment samples and tended to decrease with distance. Chemical contamination and (geno)toxic effects were greater in sediment extracts from the intertidal section than from the subtidal section. However, no evident change in chemical or toxicological characteristics was noted between samples obtained at different depths. Spearman rank-correlation analysis revealed some trends between levels of some PAHs and (geno)toxicity in hepatocytes exposed to sediment extracts.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0378-4274
pubmed:author
pubmed:issnType
Print
pubmed:volume
78
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
175-82
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Genotoxicity of sediment extracts obtained in the vicinity of a creosote-treated wharf to rainbow trout hepatocytes.
pubmed:affiliation
St. Lawrence Centre, Environment Canada, Montreal, Quebec.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't