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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5224
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pubmed:dateCreated |
1995-8-31
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pubmed:abstractText |
Eukaryotic DNA polymerase beta (pol beta) can catalyze DNA synthesis during base excision DNA repair. It is shown here that pol beta also catalyzes release of 5'-terminal deoxyribose phosphate (dRP) residues from incised apurinic-apyrimidinic sites, which are common intermediate products in base excision repair. The catalytic domain for this activity resides within an amino-terminal 8-kilodalton fragment of pol beta, which comprises a distinct structural domain of the enzyme. Magnesium is required for the release of dRP from double-stranded DNA but not from a single-stranded oligonucleotide. Analysis of the released products indicates that the excision reaction occurs by beta-elimination rather than hydrolysis.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Apurinic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Ligases,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Polymerase I,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-(Apurinic or Apyrimidinic...,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyribonuclease IV (Phage...,
http://linkedlifedata.com/resource/pubmed/chemical/Edetic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Lyases,
http://linkedlifedata.com/resource/pubmed/chemical/Polynucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Ribosemonophosphates,
http://linkedlifedata.com/resource/pubmed/chemical/apyrimidinic acid
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0036-8075
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
4
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pubmed:volume |
269
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
699-702
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:7624801-Animals,
pubmed-meshheading:7624801-Apurinic Acid,
pubmed-meshheading:7624801-Base Sequence,
pubmed-meshheading:7624801-Binding Sites,
pubmed-meshheading:7624801-DNA,
pubmed-meshheading:7624801-DNA Ligases,
pubmed-meshheading:7624801-DNA Polymerase I,
pubmed-meshheading:7624801-DNA Repair,
pubmed-meshheading:7624801-DNA-(Apurinic or Apyrimidinic Site) Lyase,
pubmed-meshheading:7624801-Deoxyribonuclease IV (Phage T4-Induced),
pubmed-meshheading:7624801-Edetic Acid,
pubmed-meshheading:7624801-Hydrolysis,
pubmed-meshheading:7624801-Lyases,
pubmed-meshheading:7624801-Molecular Sequence Data,
pubmed-meshheading:7624801-Polynucleotides,
pubmed-meshheading:7624801-Protein Structure, Tertiary,
pubmed-meshheading:7624801-Rats,
pubmed-meshheading:7624801-Ribosemonophosphates,
pubmed-meshheading:7624801-Xenopus laevis
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pubmed:year |
1995
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pubmed:articleTitle |
Excision of deoxyribose phosphate residues by DNA polymerase beta during DNA repair.
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pubmed:affiliation |
Department of Radiation Oncology, Fox Chase Cancer Center, Philadelphia, PA 19111, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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