Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-8-30
pubmed:abstractText
Surfactant proteins A and D (SP-A, SP-D) can interact with lipopolysaccharide (LPS) and stimulate alveolar macrophages. The opsonic activities of SP-A and SP-D for bacteria with different types of LPS and alveolar macrophages were investigated. In flow cytometric studies with fluorescein-labeled rough (J5) and smooth (O111) Escherichia coli and rat alveolar macrophages, SP-A enhanced binding of J5 but not O111 bacteria to macrophages. Most importantly, SP-A enhanced ingestion of J5 bacteria by alveolar macrophages and subsequent bacterial killing. Immunoelectron microscopy demonstrated that J5 bacteria, the interface between the bacterium and the outer membrane of the alveolar macrophage, and ingested bacteria were heavily labeled with SP-A. In contrast, SP-D did not mediate phagocytosis. SP-A acted as an opsonin in the phagocytosis of rough LPS-containing bacteria by alveolar macrophages, emphasizing the possible role for SP-A in the alveolar defense system.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0022-1899
pubmed:author
pubmed:issnType
Print
pubmed:volume
172
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
481-9
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:7622892-Animals, pubmed-meshheading:7622892-Binding Sites, Antibody, pubmed-meshheading:7622892-Escherichia coli, pubmed-meshheading:7622892-Glycoproteins, pubmed-meshheading:7622892-Gram-Negative Bacteria, pubmed-meshheading:7622892-Lipopolysaccharides, pubmed-meshheading:7622892-Macrophage Activation, pubmed-meshheading:7622892-Macrophages, Alveolar, pubmed-meshheading:7622892-Male, pubmed-meshheading:7622892-Microscopy, Electron, Scanning, pubmed-meshheading:7622892-Opsonin Proteins, pubmed-meshheading:7622892-Phagocytosis, pubmed-meshheading:7622892-Proteolipids, pubmed-meshheading:7622892-Pulmonary Surfactant-Associated Protein A, pubmed-meshheading:7622892-Pulmonary Surfactant-Associated Protein D, pubmed-meshheading:7622892-Pulmonary Surfactant-Associated Proteins, pubmed-meshheading:7622892-Pulmonary Surfactants, pubmed-meshheading:7622892-Rats, pubmed-meshheading:7622892-Rats, Wistar
pubmed:year
1995
pubmed:articleTitle
Opsonic activities of surfactant proteins A and D in phagocytosis of gram-negative bacteria by alveolar macrophages.
pubmed:affiliation
Eijkman-Winkler Institute for Medical Microbiology, Department of Functional Morphology, Utrecht University, Netherlands.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't