|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
1995-8-31
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
Expression of the interleukin 10-encoding (IL-10) mRNA by ovine (ov-) cells, in response to mitogenic stimulation, was assessed by Northern blot and polymerase chain reaction (PCR) analyses using a human (hu) IL-10 cDNA probe and oligodeoxyribonucleotide primers based on homologous regions of the human and murine IL-10 cDNA sequences. A 315-bp cDNA generated by the PCR analysis was cloned and used to screen a lipopolysaccharide-stimulated alveolar ov-macrophage cDNA library. The full-length ov-cDNA sequence isolated translates to a protein of 177 amino acids (aa) with a predicted 18-aa leader sequence and molecular mass of 20,165 Da. Expression in a mammalian system demonstrated that the ov-cDNA encoded a protein with the expected IL-10 biological activity. Both recombinant huIL-10 and supernatants from COS cells transfected with an expression vector containing the ovIL-10 cDNA inhibited production of IL-1 and tumour necrosis factor-alpha by ov-alveolar macrophages. Genomic DNA analysis indicated ovIL-10 exists as a single gene within the ov-genome.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jul
|
|
pubmed:issn |
0378-1119
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
4
|
|
pubmed:volume |
159
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
187-91
|
|
pubmed:dateRevised |
2004-11-17
|
|
pubmed:meshHeading |
pubmed-meshheading:7622047-Amino Acid Sequence,
pubmed-meshheading:7622047-Animals,
pubmed-meshheading:7622047-Base Sequence,
pubmed-meshheading:7622047-Blotting, Southern,
pubmed-meshheading:7622047-Cloning, Molecular,
pubmed-meshheading:7622047-Cytokines,
pubmed-meshheading:7622047-DNA, Complementary,
pubmed-meshheading:7622047-Genome,
pubmed-meshheading:7622047-Humans,
pubmed-meshheading:7622047-Interleukin-10,
pubmed-meshheading:7622047-Macrophages,
pubmed-meshheading:7622047-Molecular Sequence Data,
pubmed-meshheading:7622047-RNA, Messenger,
pubmed-meshheading:7622047-Recombinant Proteins,
pubmed-meshheading:7622047-Sequence Analysis, DNA,
pubmed-meshheading:7622047-Sequence Homology, Amino Acid,
pubmed-meshheading:7622047-Sheep,
pubmed-meshheading:7622047-Species Specificity,
pubmed-meshheading:7622047-Tissue Distribution
|
|
pubmed:year |
1995
|
|
pubmed:articleTitle |
Cloning and characterisation of an ovine interleukin-10-encoding cDNA.
|
|
pubmed:affiliation |
Centre for Animal Biotechnology, School of Veterinary Science, University of Melbourne, Parkville, Australia.
|
|
pubmed:publicationType |
Journal Article
|
