Linked Life Data

7619837

Source:http://linkedlifedata.com/resource/pubmed/id/7619837

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1995-8-30
pubmed:abstractText
Renal brush border membranes contain several anion exchanges that may play a role in electrolyte transport and pH regulation. To help characterize the types of exchangers present and the binding properties of these membranes, the binding of nitrate (NO3-) to highly purified rabbit kidney brush border membrane vesicles was studied. The method is based on the binding induced quadrupole relaxation of the 14N-NMR signal of nitrate [1,2]. Brush border membrane vesicles caused a relaxation of the 14N-NMR nitrate signal which could be characterized by relatively high affinity sites, KD = 6.7 +/- 1.5 mM, as well as nonspecific interactions with the membranes, KD > 150 mM. The anion transport inhibitor 4,4'-dinitrostilbene-2,2'-disulfonate (DNDS) inhibited 51 +/- 6% (n = 4) of the relaxation due to the high affinity binding sites. The DNDS inhibition could be characterized by a Ki of 10-80 microM. Both bicarbonate and formate (HCO2-) were found to partially inhibit the high affinity induced relaxation, with maximal inhibition of 37 +/- 8% (n = 3) and 30 +/- 2% (n = 3), respectively. The inhibitory effects of saturating concentrations of bicarbonate and formate were non-additive, suggesting the existence of a stilbene sensitive exchanger that can bind nitrate, as well as both bicarbonate and formate. This study indicates the usefulness of this new method for further investigation of anion exchangers on these and other membranes.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
6
pubmed:volume
1237
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
16-22
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Binding of nitrate to renal brush border membranes studied with 14N nuclear magnetic resonance (NMR).
pubmed:affiliation
Department of Medicine, University of Illinois College of Medicine, Chicago, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't