Linked Life Data

7619490

Source:http://linkedlifedata.com/resource/pubmed/id/7619490

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1995-8-31
pubmed:abstractText
To increase the reproducibility and to reduce the false positives in the initial mRNA differential display, modified long composite primers were developed based on both mRNA differential display and RNA arbitrarily primed PCR fingerprinting methods. Ten-base nucleotides were added at the 5' ends of the primers used in the initial mRNA differential display. These included a restriction site to aid cloning. PCR began with one low-stringency cycle (40 degrees C for annealing) followed by 35 high-stringency cycles (60 degrees C for annealing). The modified method significantly improved the reproducibility and sensitivity of the mRNA differential display while still keeping the characteristics of the original method.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0736-6205
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:geneSymbol
H-ras
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
842-6, 848, 850
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
New primer strategy improves precision of differential display.
pubmed:affiliation
Dana-Farber Cancer Institute, Boston, MA, USA.
pubmed:publicationType
Research Support, U.S. Gov't, P.H.S., Technical Report