Linked Life Data

7618264

Source:http://linkedlifedata.com/resource/pubmed/id/7618264

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-8-18
pubmed:abstractText
Experiments many years ago showed that reducing the ionic strength of the medium Sindbis virus-infected chick embryo fibroblasts were maintained prevented the release of progeny virus into the medium. It was proposed at that time that the susceptibility of virus release to inhibition by low ionic strength (LIS) medium might be determined by the properties of the viral envelope proteins. We thought that if such were the case, it might be possible to obtain a viral mutant which is resistant to the inhibitory effect of LIS medium. We report here that we were able to isolate such a mutant of Sindbis virus, and that we identified two amino acids (Glu23 and Thr76) which, when present in the E2 protein, made possible the release of infectious progeny virus from cells incubated in LIS medium.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0042-6822
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
210
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
237-43
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
A mutant of Sindbis virus which is released efficiently from cells maintained in low ionic strength medium.
pubmed:affiliation
Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway 08854-5635, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't