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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
1995-8-23
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pubmed:abstractText |
The relationship between the initial increase of intracellular Ca2+ concentration ([Ca2+]i) (measured at the single-cell level with an imaging system) and the ensuing proliferation was examined in a human T cell clone stimulated by a phorbol ester in combination with ionomycin, thapsigargin or an anti-CD3 mAb (monoclonal antibody against the CD3 molecule, UCHT1). From the responses to various ionomycin concentrations, one can define a range of [Ca2+]i values (400-900 nM) which appears optimal for T cell proliferation; lower [Ca2+]i values are suboptimal, higher values are cytotoxic. It was then examined if the [Ca2+]i requirements were similar following anti-CD3 stimulation. [Ca2+]i oscillations elicited by a concentration of UCHT1 (1/1,000) optimal for mitogenicity fall precisely within the 400-900 nM range. However, very low concentrations of UCHT1 (1/100,000) which evoke barely detectable [Ca2+]i responses still cause the cells to proliferate. The possibility that the lower [Ca2+]i requirements observed following anti-CD3 stimulation was due to [Ca2+]i oscillations was tested under conditions which prevented the appearance of these oscillations. It turns out that an oscillatory Ca2+ signal is not more mitogenic than a sustained augmentation of [Ca2+]i. Finally, it was examined if overstimulation via CD3 could have toxic consequences similar to those elicited after ionomycin overstimulation. Large transient [Ca2+]i responses can be observed following anti-CD3 stimulation in appropriate conditions, and namely in T cells pretreated with interleukin-2. These [Ca2+]i augmentations are not cytotoxic. A role for the plasmalemmal Ca2+ pump in the prevention of cytotoxicity can be demonstrated.(ABSTRACT TRUNCATED AT 250 WORDS)
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0031-6768
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
429
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
546-54
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7617445-Antibodies,
pubmed-meshheading:7617445-Calcium,
pubmed-meshheading:7617445-Cells, Cultured,
pubmed-meshheading:7617445-Dose-Response Relationship, Drug,
pubmed-meshheading:7617445-Egtazic Acid,
pubmed-meshheading:7617445-Humans,
pubmed-meshheading:7617445-Ionomycin,
pubmed-meshheading:7617445-T-Lymphocytes,
pubmed-meshheading:7617445-Time Factors
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pubmed:year |
1995
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pubmed:articleTitle |
The intracellular Ca2+ concentration optimal for T cell activation is quite different after ionomycin or CD3 stimulation.
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pubmed:affiliation |
Laboratoire de Neurobiologie, CNRS URA 1857, Ecole Normale Supérieure, Paris, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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