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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6537
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pubmed:dateCreated |
1995-8-22
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pubmed:abstractText |
Affinity maturation of antibodies is characterized by localized hypermutation of the DNA around the V segment. Here we show, using mice containing single or multiple transgene constructs, that an immunoglobulin V kappa segment can be replaced by human beta-globin or prokaryotic neo or gpt genes without affecting the rate of hypermutation; the V gene itself is not necessary for recruiting hypermutation. The ability to target hypermutation to heterologous genes in vivo could find more general applications in biology.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Escherichia coli Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Globins,
http://linkedlifedata.com/resource/pubmed/chemical/Gpt protein, E coli,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Variable Region,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin kappa-Chains,
http://linkedlifedata.com/resource/pubmed/chemical/Kanamycin Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/Pentosyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphotransferases (Alcohol Group...,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0028-0836
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
376
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pubmed:geneSymbol |
gpt,
neo
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
225-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7617031-Animals,
pubmed-meshheading:7617031-Bacterial Proteins,
pubmed-meshheading:7617031-Base Sequence,
pubmed-meshheading:7617031-DNA Mutational Analysis,
pubmed-meshheading:7617031-DNA Primers,
pubmed-meshheading:7617031-Escherichia coli Proteins,
pubmed-meshheading:7617031-Globins,
pubmed-meshheading:7617031-Humans,
pubmed-meshheading:7617031-Hybridomas,
pubmed-meshheading:7617031-Immunoglobulin Variable Region,
pubmed-meshheading:7617031-Immunoglobulin kappa-Chains,
pubmed-meshheading:7617031-Kanamycin Kinase,
pubmed-meshheading:7617031-Mice,
pubmed-meshheading:7617031-Mice, Transgenic,
pubmed-meshheading:7617031-Molecular Sequence Data,
pubmed-meshheading:7617031-Mutagenesis,
pubmed-meshheading:7617031-Pentosyltransferases,
pubmed-meshheading:7617031-Peyer's Patches,
pubmed-meshheading:7617031-Phosphotransferases (Alcohol Group Acceptor),
pubmed-meshheading:7617031-Proteins,
pubmed-meshheading:7617031-Recombinant Fusion Proteins
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pubmed:year |
1995
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pubmed:articleTitle |
Targeting of non-Ig sequences in place of the V segment by somatic hypermutation.
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pubmed:affiliation |
Medical Research Council Laboratory of Molecular Biology, Cambridge, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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