Linked Life Data

7615794

Source:http://linkedlifedata.com/resource/pubmed/id/7615794

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1995-8-24
pubmed:abstractText
Using phosphorimager technology to quantitate differences in protein expression, we have investigated the modulation of protein synthesis by Mycobacterium tuberculosis in response to intracellular residence in human macrophages and, for comparison, in response to various stress conditions during extracellular growth. Proteins of M. tuberculosis growing intracellularly in human THP-1 cells and extracellularly in broth were labeled with [35S]methionine; during intracellular growth, host cell protein synthesis was inhibited with cycloheximide. The metabolically labeled proteins were separated by two-dimensional gel electrophoresis and quantitatively analyzed. Intracellular residence in macrophages induced a profound change in the overall phenotype of M. tuberculosis. The expression of at least 16 M. tuberculosis proteins was induced (at least a twofold increase compared with growth in broth) and 28 proteins repressed (at least a twofold decrease). Many of the phenotypic changes in protein expression induced during intracellular growth occurred during extracellular growth in response to stress conditions including heat-shock, low pH, and H2O2. However, the pattern of induced and repressed proteins was unique to each stress condition. Of the 16 macrophage-induced proteins, 6 were absent during extracellular growth under both normal and stress conditions. Such proteins are potential virulence determinants and/or they may be important in the cell-mediated and protective immune response to M. tuberculosis infection.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7615794-1398989, http://linkedlifedata.com/resource/pubmed/commentcorrection/7615794-1601032, http://linkedlifedata.com/resource/pubmed/commentcorrection/7615794-1616212, http://linkedlifedata.com/resource/pubmed/commentcorrection/7615794-1882440, http://linkedlifedata.com/resource/pubmed/commentcorrection/7615794-1898911, http://linkedlifedata.com/resource/pubmed/commentcorrection/7615794-1970672, http://linkedlifedata.com/resource/pubmed/commentcorrection/7615794-2108212, http://linkedlifedata.com/resource/pubmed/commentcorrection/7615794-236308, http://linkedlifedata.com/resource/pubmed/commentcorrection/7615794-2642524, http://linkedlifedata.com/resource/pubmed/commentcorrection/7615794-3316470, http://linkedlifedata.com/resource/pubmed/commentcorrection/7615794-6949641, http://linkedlifedata.com/resource/pubmed/commentcorrection/7615794-6970727, http://linkedlifedata.com/resource/pubmed/commentcorrection/7615794-7190579, http://linkedlifedata.com/resource/pubmed/commentcorrection/7615794-7807006, http://linkedlifedata.com/resource/pubmed/commentcorrection/7615794-7878014, http://linkedlifedata.com/resource/pubmed/commentcorrection/7615794-8454334
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0021-9738
pubmed:author
pubmed:issnType
Print
pubmed:volume
96
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
245-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Identification of macrophage and stress-induced proteins of Mycobacterium tuberculosis.
pubmed:affiliation
Department of Medicine, School of Medicine, University of California, Los Angeles 90095, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.