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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1995-8-24
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pubmed:abstractText |
Changes in intracellular pH (pHin) take part in the mitogenic response. Their importance has been stressed by the finding that mouse fibroblasts expressing a yeast proton pumping ATPase (PMA1) exhibit a transformed phenotype and are tumorigenic. These cells do maintain a higher pHin, supporting the idea that elevated pHin may act as a proliferative trigger. Here we show that cells constitutively expressing PMA1 have higher levels of the AP-1 transcription factor. The use of stable transfectants and transient transfection assays show that PMA1 activity induces transactivation of the c-fos promoter. The activation of the promoter is mediated throughout the serum response element (SRE). The use of protein kinase C inhibitors suggests that AP-1 activation is achieved through a pathway independent of protein kinase C.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphatases,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP-Dependent Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Endopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Forskolin,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oncogene Proteins, Fusion,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proton Pumps,
http://linkedlifedata.com/resource/pubmed/chemical/Serum Response Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Tissue Plasminogen Activator,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor AP-1,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/USP6 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Ubiquitin Thiolesterase
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0730-2312
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
57
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pubmed:geneSymbol |
4XTRE,
PMA1,
c-fos
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
630-40
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:7615647-3T3 Cells,
pubmed-meshheading:7615647-Adenosine Triphosphatases,
pubmed-meshheading:7615647-Animals,
pubmed-meshheading:7615647-Base Sequence,
pubmed-meshheading:7615647-Cattle,
pubmed-meshheading:7615647-Culture Media,
pubmed-meshheading:7615647-Cyclic AMP-Dependent Protein Kinases,
pubmed-meshheading:7615647-DNA-Binding Proteins,
pubmed-meshheading:7615647-Electrophoresis,
pubmed-meshheading:7615647-Endopeptidases,
pubmed-meshheading:7615647-Forskolin,
pubmed-meshheading:7615647-Genes, fos,
pubmed-meshheading:7615647-Hydrogen-Ion Concentration,
pubmed-meshheading:7615647-Mice,
pubmed-meshheading:7615647-Molecular Sequence Data,
pubmed-meshheading:7615647-Nuclear Proteins,
pubmed-meshheading:7615647-Oncogene Proteins,
pubmed-meshheading:7615647-Oncogene Proteins, Fusion,
pubmed-meshheading:7615647-Promoter Regions, Genetic,
pubmed-meshheading:7615647-Protein Kinase C,
pubmed-meshheading:7615647-Protein Kinase Inhibitors,
pubmed-meshheading:7615647-Proto-Oncogene Proteins,
pubmed-meshheading:7615647-Proton Pumps,
pubmed-meshheading:7615647-Serum Response Factor,
pubmed-meshheading:7615647-Tissue Plasminogen Activator,
pubmed-meshheading:7615647-Transcription Factor AP-1,
pubmed-meshheading:7615647-Transcription Factors,
pubmed-meshheading:7615647-Transcriptional Activation,
pubmed-meshheading:7615647-Transfection,
pubmed-meshheading:7615647-Ubiquitin Thiolesterase
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pubmed:year |
1995
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pubmed:articleTitle |
Activation of the c-fos promoter by increased internal pH.
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pubmed:affiliation |
Instituto de Investigaciones Biomédicas del C.S.I.C., Madrid, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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