Linked Life Data

7614370

Source:http://linkedlifedata.com/resource/pubmed/id/7614370

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1995-8-18
pubmed:abstractText
Interest in extracellular fungal lipases has increased mainly because of their industrial applications. However, no studies have been done on a genetically well characterized filamentous fungus like Aspergillus nidulans. Here we show that A. nidulans produces an extracellular lipase when grown in solid or liquid cultures containing lipids as carbon source. This lipase is glucose-repressed in a creA-independent fashion. Seven mutants isolated by their inability to utilize lipids as sole carbon source were also unable to utilize acetate as sole carbon source. Representative mutants from each of three complementation groups were tested for allelism with strains carrying well known mutations affecting acetate metabolism. They were found to contain acuD (Isocitrate lyase), acuF (PEP carboxykinase), and acuE (Malate synthase) alleles. Screening of lipid nonutilizing mutants for growth in acetate provides a method for the isolation of both lipase minus and new acetate metabolism mutants.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0147-5975
pubmed:author
pubmed:issnType
Print
pubmed:volume
19
pubmed:geneSymbol
acuD, acuE, acuF
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
81-5
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Aspergillus nidulans mutants affected in acetate metabolism isolated as lipid nonutilizers.
pubmed:affiliation
Departamento de Alimentos y Biotecnología, Facultad de Química, UNAM, México, D.F.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't