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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1995-8-18
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pubmed:abstractText |
The ultrastructure of isolated cell walls of Schizosaccharomyces pombe was studied by electron microscopy after treatment with the following purified enzymes: endo-beta-(1-->3)-glucanase, endo-beta-(-->6)-glucanase, and endo-alpha-(1-->3)-glucanase produced by Bacillus circulans; exo-beta-(1-->3)-glucanase and endo-beta-(1-->3)-glucanase produced by Schizosaccharomyces japonicus var. versatilis. The exo-beta-(1-->3)-glucanase had no detectable effect on the walls, but amorphous wall material was removed by action of the endo-beta-(1-->3)- and endo-beta-(1-->6)-glucanases of B. circulans to reveal a wall component consisting of densely interwoven microfibrils. The fibrils were hydrolyzed by treatment with the Schiz. japonicus endo-beta-(1-->3)-glucanase followed by B. circulans endo-alpha-(1-->3)-glucanase--suggesting that they were composed of -beta-(1-->3)-linked glucan and alpha-(1-->3)-linked glucan. The presence of a fibrillar component in untreated walls was evident after negative staining.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Glucan 1,3-beta-Glucosidase,
http://linkedlifedata.com/resource/pubmed/chemical/Glucans,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoside Hydrolases,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Glucosidase,
http://linkedlifedata.com/resource/pubmed/chemical/endo-1,6-beta-glucanase,
http://linkedlifedata.com/resource/pubmed/chemical/exo-1,3-alpha-glucanase
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pubmed:status |
MEDLINE
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pubmed:issn |
1047-8477
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
114
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
140-52
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:7612397-Actin Cytoskeleton,
pubmed-meshheading:7612397-Cell Wall,
pubmed-meshheading:7612397-Glucan 1,3-beta-Glucosidase,
pubmed-meshheading:7612397-Glucans,
pubmed-meshheading:7612397-Glycoside Hydrolases,
pubmed-meshheading:7612397-Microscopy, Electron,
pubmed-meshheading:7612397-Schizosaccharomyces,
pubmed-meshheading:7612397-beta-Glucosidase
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pubmed:articleTitle |
Ultrastructure of the cell wall of Schizosaccharomyces pombe following treatment with various glucanases.
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pubmed:affiliation |
Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
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pubmed:publicationType |
Journal Article
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