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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6 Pt 1
|
| pubmed:dateCreated |
1995-8-15
|
| pubmed:abstractText |
Reactive oxygen species play an important role in pathogenesis of a variety of pathological processes, e.g., ischemia-reperfusion, acute viral infections, thermal injury, hepatic diseases, and acute lung injury. Xanthine oxidase (XO) may be a significant source of these cytotoxic oxygen species. We tested the hypothesis that hepatic ischemia-reperfusion releases xanthine dehydrogenase + XO (XDH + XO) into the circulation and that circulating XO damages isolated perfused lung. Isolated liver + lung preparation was perfused with Krebs-Henseleit buffer to minimize confounding effects of circulating neutrophils. In one group, livers were rendered globally ischemic for 2 h and then reperfused (I/R). In another group, livers were pretreated with allopurinol and perfused with buffer containing additional allopurinol (I/R + Allo). After 2 h of ischemia, an isolated lung was connected to liver, and liver + lung preparation was reperfused in series for 15 min. Liver reperfusion was terminated, and lung was recirculated with liver effluent for 45 min. Capillary filtration coefficient (ml.min-1.cmH2O-1.100 g lung dry wt-1) was 2.0 +/- 0.3 and 1.9 +/- 0.4 in control and I/R + Allo lungs, respectively, and 9.0 +/- 1.2 in I/R lungs (P < 0.001). Lung wet-to-dry weight ratio in control and I/R + Allo lungs was 8.6 +/- 0.3 and 9.1 +/- 0.5, respectively, and 14.9 +/- 1.1 in I/R lungs (P < 0.01). Control and I/R + Allo bronchoalveolar lavage protein content was < 1.0 mg/ml compared with 32.6 +/- 8.4 mg/ml in I/R group.(ABSTRACT TRUNCATED AT 250 WORDS)
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Allopurinol,
http://linkedlifedata.com/resource/pubmed/chemical/L-Lactate Dehydrogenase,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Reactive Oxygen Species,
http://linkedlifedata.com/resource/pubmed/chemical/Xanthine Dehydrogenase,
http://linkedlifedata.com/resource/pubmed/chemical/Xanthine Oxidase
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0002-9513
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
268
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
G988-96
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7611420-Allopurinol,
pubmed-meshheading:7611420-Animals,
pubmed-meshheading:7611420-Bronchoalveolar Lavage Fluid,
pubmed-meshheading:7611420-Ischemia,
pubmed-meshheading:7611420-L-Lactate Dehydrogenase,
pubmed-meshheading:7611420-Liver,
pubmed-meshheading:7611420-Liver Circulation,
pubmed-meshheading:7611420-Lung,
pubmed-meshheading:7611420-Male,
pubmed-meshheading:7611420-Perfusion,
pubmed-meshheading:7611420-Permeability,
pubmed-meshheading:7611420-Proteins,
pubmed-meshheading:7611420-Pulmonary Circulation,
pubmed-meshheading:7611420-Rats,
pubmed-meshheading:7611420-Rats, Sprague-Dawley,
pubmed-meshheading:7611420-Reactive Oxygen Species,
pubmed-meshheading:7611420-Reperfusion,
pubmed-meshheading:7611420-Xanthine Dehydrogenase,
pubmed-meshheading:7611420-Xanthine Oxidase
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Liver ischemia-reperfusion increases pulmonary permeability in rat: role of circulating xanthine oxidase.
|
| pubmed:affiliation |
Department of Anesthesiology, University of Alabama at Birmingham 35233, USA.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|