Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1995-8-11
pubmed:abstractText
The Epstein-Barr virus BamHI F promoter (Fp) was previously identified as the putative EBNA 1 gene promoter in group 1 Burkitt's lymphoma (BL) cell lines. Fp has also been shown to be activated in Epstein-Barr virus-positive B-cell lines following induction of the viral productive cycle (A. L. Lear, M. Rowe, M. G. Kurilla, S. Lee, S. Henderson, E. Kieff, and A. B. Rickinson, J. Virol. 66:7461-7468, 1992). Here we demonstrate that Fp is exclusively a lytic promoter which was incorrectly identified as the EBNA 1 gene promoter in group 1 BL cell lines. It is shown that while Fp activity was observed in two group 1 BL cell lines, it could not be detected in a third group 1 BL cell line. Furthermore, the level of Fp activity detected in both group 1 and group 3 cell lines appeared to correlate only with the level of spontaneous lytic activity. Induction of the lytic cycle in group 1 or group 3 BL cell lines resulted in a dramatic increase in Fp-initiated transcripts but no detectable increase in EBNA 1 transcripts. Anti-immunoglobulin induction of the lytic cycle in the Akata group 1 BL cell line revealed that induction of Fp activity was detectable by 2 to 4 h after induction of the lytic cycle and was dependent on de novo protein synthesis. In addition, Fp reporter constructs transiently transfected into group 1 BL cell lines exhibited activity which was independent of the Fp initiation site, TATAA box, or other upstream sequences. The sequences required for efficient reporter gene activity mapped to a region ca. 210 bp downstream of the Fp cap site. Furthermore, Northern (RNA) blot analyses indicated that there are two Fp-initiated lytic transcripts between 9 and 15 kb in size, neither of which correspond to the known EBNA 1 transcripts present in group 1 BL cell lines.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7609074-1309242, http://linkedlifedata.com/resource/pubmed/commentcorrection/7609074-1310556, http://linkedlifedata.com/resource/pubmed/commentcorrection/7609074-1321268, http://linkedlifedata.com/resource/pubmed/commentcorrection/7609074-1331531, http://linkedlifedata.com/resource/pubmed/commentcorrection/7609074-1370554, http://linkedlifedata.com/resource/pubmed/commentcorrection/7609074-1647567, http://linkedlifedata.com/resource/pubmed/commentcorrection/7609074-1648738, http://linkedlifedata.com/resource/pubmed/commentcorrection/7609074-1658397, http://linkedlifedata.com/resource/pubmed/commentcorrection/7609074-1713681, http://linkedlifedata.com/resource/pubmed/commentcorrection/7609074-2155423, http://linkedlifedata.com/resource/pubmed/commentcorrection/7609074-2165133, http://linkedlifedata.com/resource/pubmed/commentcorrection/7609074-2440339, http://linkedlifedata.com/resource/pubmed/commentcorrection/7609074-2462063, http://linkedlifedata.com/resource/pubmed/commentcorrection/7609074-2549539, http://linkedlifedata.com/resource/pubmed/commentcorrection/7609074-2822952, http://linkedlifedata.com/resource/pubmed/commentcorrection/7609074-2824192, http://linkedlifedata.com/resource/pubmed/commentcorrection/7609074-3023050, http://linkedlifedata.com/resource/pubmed/commentcorrection/7609074-3025831, http://linkedlifedata.com/resource/pubmed/commentcorrection/7609074-3460083, http://linkedlifedata.com/resource/pubmed/commentcorrection/7609074-4122458, http://linkedlifedata.com/resource/pubmed/commentcorrection/7609074-4504350, http://linkedlifedata.com/resource/pubmed/commentcorrection/7609074-6154876, http://linkedlifedata.com/resource/pubmed/commentcorrection/7609074-6960240, http://linkedlifedata.com/resource/pubmed/commentcorrection/7609074-7831773, http://linkedlifedata.com/resource/pubmed/commentcorrection/7609074-8057484, http://linkedlifedata.com/resource/pubmed/commentcorrection/7609074-8164660
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
69
pubmed:geneSymbol
EBNA 1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5039-47
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:7609074-Animals, pubmed-meshheading:7609074-Antigens, Viral, pubmed-meshheading:7609074-Base Sequence, pubmed-meshheading:7609074-Blotting, Northern, pubmed-meshheading:7609074-Burkitt Lymphoma, pubmed-meshheading:7609074-Cell Line, pubmed-meshheading:7609074-DNA-Binding Proteins, pubmed-meshheading:7609074-Deoxyribonuclease BamHI, pubmed-meshheading:7609074-Epstein-Barr Virus Nuclear Antigens, pubmed-meshheading:7609074-Exons, pubmed-meshheading:7609074-Herpesvirus 4, Human, pubmed-meshheading:7609074-Molecular Sequence Data, pubmed-meshheading:7609074-Oligodeoxyribonucleotides, pubmed-meshheading:7609074-Phenotype, pubmed-meshheading:7609074-Promoter Regions, Genetic, pubmed-meshheading:7609074-RNA, Messenger, pubmed-meshheading:7609074-Sequence Deletion, pubmed-meshheading:7609074-Single-Strand Specific DNA and RNA Endonucleases, pubmed-meshheading:7609074-Transcription, Genetic, pubmed-meshheading:7609074-Tumor Cells, Cultured
pubmed:year
1995
pubmed:articleTitle
The Epstein-Barr virus BamHI F promoter is an early lytic promoter: lack of correlation with EBNA 1 gene transcription in group 1 Burkitt's lymphoma cell lines.
pubmed:affiliation
Division of Tumor Virology, Dana-Farber Cancer Institute, Boston, Massachusetts 02216, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't