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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
27
|
| pubmed:dateCreated |
1995-8-16
|
| pubmed:abstractText |
The B creatine kinase gene is regulated by an array of positive and negative cis-elements in the 5'-flanking DNA that function in both muscle and nonmuscle cells. In C2C12 myogenic cells M and B creatine kinase mRNAs are coordinately up-regulated in the early stages of myogenesis and then undergo distinct regulatory programs. The B creatine kinase gene is down-regulated in the late stages of myogenesis as M creatine kinase becomes the predominant species in mature myotubes. Sequences between -92 and +80 of the B creatine kinase gene confer a regulated pattern of expression to chimeric plasmids that closely resembles the time-course of expression of the endogenous B creatine kinase gene in C2C12 cells undergoing differentiation. We show that sequences within the first exon of the B creatine kinase gene are important for the development regulation of the gene in C2C12 cells and that these sequences bind a nuclear protein that shows a similar tissue-specific distribution and developmentally regulated expression to that of the endogenous B creatine kinase gene.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
7
|
| pubmed:volume |
270
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
16134-9
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:7608177-Animals,
pubmed-meshheading:7608177-Blotting, Northern,
pubmed-meshheading:7608177-Cells, Cultured,
pubmed-meshheading:7608177-Creatine Kinase,
pubmed-meshheading:7608177-DNA-Binding Proteins,
pubmed-meshheading:7608177-Exons,
pubmed-meshheading:7608177-Gene Expression Regulation,
pubmed-meshheading:7608177-Genes, Reporter,
pubmed-meshheading:7608177-Humans,
pubmed-meshheading:7608177-Mice,
pubmed-meshheading:7608177-Muscle Proteins,
pubmed-meshheading:7608177-Muscles,
pubmed-meshheading:7608177-Nuclear Proteins,
pubmed-meshheading:7608177-Phosphorylation,
pubmed-meshheading:7608177-Protein Binding,
pubmed-meshheading:7608177-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:7608177-Stem Cells,
pubmed-meshheading:7608177-Transfection
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Characterization of a nuclear protein that interacts with regulatory elements in the human B creatine kinase gene.
|
| pubmed:affiliation |
Cardiovascular Division, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
|
| pubmed:publicationType |
Journal Article
|